Screening for p53 Gene Mutations in Archived Tumors of Workers Occupationally Exposed to Carcinogens: Examples from Analysis of Bladder Tumors
| Autor: | Ruggero Montesano, Paolo Vineis, Therese Sørlie, Asunción Esteve, Inca Kusters, Ghysiaine Martel-Planche, Jürgen Lewalter, Monica Hollstein, Manfred Stephan-Odenthal |
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| Rok vydání: | 1995 |
| Předmět: |
Pathology
medicine.medical_specialty Tumor suppressor gene DNA Mutational Analysis Molecular Sequence Data Gene mutation Bioinformatics Polymerase Chain Reaction Genetic analysis Risk Factors Occupational Exposure Humans Medicine Carcinogen Urinary bladder Base Sequence Oncogene business.industry Point mutation Public Health Environmental and Occupational Health DNA Neoplasm Genes p53 medicine.anatomical_structure Urinary Bladder Neoplasms Mutation Carcinogens Occupational exposure business |
| Zdroj: | Journal of Occupational and Environmental Medicine. 37:59-68 |
| ISSN: | 1076-2752 |
| DOI: | 10.1097/00043764-199501000-00009 |
| Popis: | Point mutations in the p53 tumor suppressor gene are the most common genetic alterations in human cancers. The nature and location of these mutations can be informative in assessing the importance of putative carcinogenic agents. Potential associations between a given carcinogen and a specific mutation pattern can be substantiated when the exposure history of the patients is known. While the past exposure to environmental risk factors is often difficult to determine, documented occupational exposure to carcinogens presents a unique situation for evaluating this approach. Analysis usually involves working with paraffin-embedded tissues, fixed under conditions suboptimal for genetic analysis and stored for many years, since frozen tissues are not available in sufficient numbers. The particular methodological problems encountered with fixed samples are discussed here, using as illustration an ongoing study of oncogene and tumor suppressor gene mutations in archived bladder tumors of workers exposed to aromatic amines and nonexposed patients. |
| Databáze: | OpenAIRE |
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