H pyloriinfection causes chronic pancreatitis in Mongolian gerbils
| Autor: | Juanita L. Merchant, Rainer Haas, Gabriele Rieder, Arno Karnholz, Mechthild Stoeckelhuber |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial medicine.medical_specialty animal diseases medicine.medical_treatment Radioimmunoassay Biology Gerbil Gastroenterology Helicobacter Infections Internal medicine parasitic diseases Gastrins medicine Animals Trichrome stain RNA Messenger Antrum Gastrin Helicobacter pylori H Pylori Lipase General Medicine Hydrogen-Ion Concentration medicine.disease Glucose medicine.anatomical_structure Endocrinology Cytokine Pancreatitis Amylases Cytokines Female sense organs Gastritis medicine.symptom Gerbillinae Pancreas |
| Zdroj: | World Journal of Gastroenterology. 13:3939 |
| ISSN: | 1007-9327 |
| Popis: | AIM: To investigate whether chronic H pylori infection has the potential to induce pancreatitis in the Mongolian gerbil model, and whether it is dependent on an intact type Ⅳ secretion system. METHODS: Mongolian gerbils were infected with wild type (WT) H pylori typeⅠstrain B128 or its isogenic mutant B128 ΔcagY (defective type Ⅳ secretion). After seven months of infection, H pylori was reisolated from antrum and corpus and H pylori DNA was analyzed by seminested polymerase chain reaction (PCR). Inflammation and histological changes were documented in the gastric antrum, corpus, and pancreas by immunohistochemistry. Cytokine mRNA, gastric pH, plasma gastrin, amylase, lipase, and glucose levels were determined. RESULTS: The H pylori infection rate was 95%. Eight infected animals, but none of the uninfected group, developed transmural inflammation and chronic pancreatitis. Extensive interstitial fibrosis and inflammation of the pancreatic lobe adjacent to the antrum was confi rmed by trichrome stain, and immunohistochemically. Pro-inflammatory cytokine mRNA was significantly increased in the antral mucosa of all infected gerbils. In the corpus, only cytokine levels of WT-infected animals and those developing transmural infl ammation and pancreatitis were signifi cantly increased. Levels of lipase, but not glucose or amylase levels, were significantly reduced in the pancreatitis group. H pylori DNA was detected in infected antral and corpus tissue, but not in the pancreas. CONCLUSION: H pylori infection is able to induce chronic pancreatitis in Mongolian gerbils independently of the type Ⅳ secretion system, probably by an indirect mechanism associated with a penetrating ulcer. |
| Databáze: | OpenAIRE |
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