Evaluation of an osteochondral fragment–groove procedure for induction of metacarpophalangeal joint osteoarthritis in horses
| Autor: | Alicia L. Bertone, Luc Duchateau, Jan H. Spaas, Leen Van Brantegem, Ann Martens, Sarah Broeckx, Frederik Pille, Koen Chiers, Maarten Oosterlinck, Simon Buntinx |
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| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular Male 040301 veterinary sciences Lameness Animal Osteoarthritis Metacarpophalangeal Joint 0403 veterinary science Gross examination Synovitis Synovial Fluid Animals Medicine Synovial fluid Horses Hyaluronic Acid Gait General Veterinary business.industry Synovial Membrane 0402 animal and dairy science Horse 04 agricultural and veterinary sciences General Medicine Metacarpophalangeal joint medicine.disease 040201 dairy & animal science Disease Models Animal medicine.anatomical_structure Lameness Female Horse Diseases Forelimb business Nuclear medicine |
| Zdroj: | American Journal of Veterinary Research. 80:246-258 |
| ISSN: | 0002-9645 |
| Popis: | OBJECTIVE To evaluate lameness and morphological changes associated with an osteochondral fragment–groove procedure as a means of experimental induction of metacarpophalangeal (MCP) joint osteoarthritis within an 11-week period in horses. ANIMALS 6 nonlame adult warmbloods. PROCEDURES The right MCP joint of each horse underwent an osteochondral fragment–groove procedure (day 0). After 1 week of stall rest (ie, starting day 7), each horse was trained daily on a treadmill. Weekly, horses underwent visual and inertial sensor-based assessments of lameness. Both MCP joints were assessed radiographically on days 0 (before surgery), 1, 35, and 77. A synovial fluid sample was collected from the right MCP joint on days 0 (before surgery), 35, 36, 49, 63, and 77 for cytologic and biomarker analyses. On day 77, each horse was euthanized; both MCP joints were evaluated macroscopically and histologically. RESULTS Right forelimb lameness was detected visually and by the inertial sensor system when horses were moving on a straight line after distal forelimb flexion or circling left on days 14 to 77. Compared with presurgical values, synovial fluid interleukin-6, prostaglandin E2, hyaluronic acid, and interleukin-1 receptor antagonist protein concentrations were increased at 2 or 3 time points, whereas tumor necrosis factor-α and interleukin-10 concentrations were decreased at 1 time point. Gross examination of all right MCP joints revealed synovitis and wear lines; synovitis was confirmed histologically. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that a combined osteochondral fragment–groove procedure can be used to induce clinically and grossly observable early MCP joint osteoarthritis during an 11-week period in horses. |
| Databáze: | OpenAIRE |
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