High-order self-assembly of G-quadruplex DNA: Nano-network formation under the guidance of arene ruthenium(II) complexes
| Autor: | Gengnan Yu, Siyan Liao, Jian Wu, Qiong Wu, Wenjie Mei |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Circular dichroism
010405 organic chemistry Phenanthroline Stacking chemistry.chemical_element DNA 010402 general chemistry G-quadruplex 01 natural sciences Biochemistry Ruthenium 0104 chemical sciences Inorganic Chemistry G-Quadruplexes chemistry.chemical_compound Crystallography chemistry Coordination Complexes Imidazole Molecule |
| Zdroj: | Journal of inorganic biochemistry. 189 |
| ISSN: | 1873-3344 |
| Popis: | Controlled self-assembly of DNA has been under intensive focus owing to its potential utility in the nano-gene device, gene therapy, and targeted drug delivery. Herein, high-order nano-network from self-assembly of c-MYC (oncogene c-MYC promoter pu22 sequences) and c-KIT1 (oncogene c-KIT1 promoter pu27 sequences) have successfully achieved G-quadruplex DNA structure when guided by arene ruthenium(II) complex, RAP-11 ([Ru(η 6 -cymene)( p -ClPIP)Cl]Cl, p -ClPIP: 2-(4-Chlorophenyl)imidazole[4,5 f ][1,10]-phenanthroline). This self-assembled nano-network structure was confirmed by AFM (atomic force microscopy) and AUC (analytical ultracentrifugation), as well as apparently induced CD (circular dichroism) signals at about 350 nm in the CD spectra of corresponding the G-quadruplex DNA. Further studies showed that the tail structure, with RAP-11 inserted into the minor groove of the G-quadruplex DNA and the head of the molecule extruded from the G-quadruplex structure, can promote head-to-head stacking through π-π stacking and play a key role in guiding well-ordered arrangement of G-quadruplexes thereby forming nano-network structure effectively. |
| Databáze: | OpenAIRE |
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