The New Age of Cell-Free Biology
| Autor: | Allen P. Liu, Vincent Noireaux |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Transcription
Genetic Biomedical Engineering Medicine (miscellaneous) Bioengineering 02 engineering and technology Cell free Computational biology Microtubules Domain (software engineering) 03 medical and health sciences Circadian Clocks Nucleic Acids Humans Gene Regulatory Networks Cytoskeleton 030304 developmental biology 0303 health sciences Cell-Free System Gene Expression Profiling Proteins 021001 nanoscience & nanotechnology Actins Variety (cybernetics) Nanostructures Protein Biosynthesis Human genome Synthetic Biology 0210 nano-technology |
| Zdroj: | Annual review of biomedical engineering. 22 |
| ISSN: | 1545-4274 |
| Popis: | The cell-free molecular synthesis of biochemical systems is a rapidly growing field of research. Advances in the Human Genome Project, DNA synthesis, and other technologies have allowed the in vitro construction of biochemical systems, termed cell-free biology, to emerge as an exciting domain of bioengineering. Cell-free biology ranges from the molecular to the cell-population scales, using an ever-expanding variety of experimental platforms and toolboxes. In this review, we discuss the ongoing efforts undertaken in the three major classes of cell-free biology methodologies, namely protein-based, nucleic acids–based, and cell-free transcription–translation systems, and provide our perspectives on the current challenges as well as the major goals in each of the subfields. |
| Databáze: | OpenAIRE |
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