Elevated levels of macromolecular damage are correlated with increased nitric oxide synthase expression in erythrocytes isolated from twin neonates
| Autor: | Edit Hermesz, Renáta Lázár, Hajnalka Orvos, Petra Talapka, Ágnes Ferencz, Krisztina N. Dugmonits, Szabolcs Zahorán |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
0301 basic medicine Erythrocytes Nitric Oxide Synthase Type III Birth weight Twins Gene Expression Gestational Age Nitric Oxide medicine.disease_cause Antioxidants Andrology Young Adult 03 medical and health sciences chemistry.chemical_compound Pregnancy medicine Birth Weight Humans Hydrogen peroxide chemistry.chemical_classification Reactive oxygen species Glutathione Disulfide 030102 biochemistry & molecular biology biology Cell Membrane Infant Newborn Hematology Metabolism medicine.disease Enzyme Activation Nitric oxide synthase Oxidative Stress 030104 developmental biology chemistry Biochemistry biology.protein Female Lipid Peroxidation Nitric Oxide Synthase Reactive Oxygen Species Oxidation-Reduction Oxidative stress Peroxynitrite |
| Zdroj: | British Journal of Haematology. 174:932-941 |
| ISSN: | 0007-1048 |
| DOI: | 10.1111/bjh.14156 |
| Popis: | Summary Pregnancy is a state associated with an enhanced metabolism and demand for O2, which may lead to the overproduction of reactive oxygen species (ROS) and hence to oxidative stress. An elevated ROS level may result in delayed development and a low birth weight. The aim of this study was to reveal the consequences of multiple pregnancies on the redox status of neonatal human red blood cells (RBCs) and evaluate the role of endothelial nitric oxide synthase (NOS3) – expressing RBCs in the generation of oxidative stress. The study presents evidence of higher levels of production of hydrogen peroxide, peroxynitrite and nitrate content in the RBCs of twin neonates, clearly reflected by an elevated level of protein and lipid damages. This phenotype appears to be a consequence of multiple pregnancies, regardless of the level of maturity or the birth weight of the twins. Besides the higher level of ROS, there was a general decrease in the expression of genes coding for antioxidants. The first data are presented on NOS3-expressing neonatal human RBCs. The number of RBCs producing NOS3 was more than twice as high in twin neonates compared to singletons, with no correlation to maturity. |
| Databáze: | OpenAIRE |
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