Measuring the activity and structure of functional RNAs inside compartments formed by liquid-liquid phase separation

Autor:	McCauley O. Meyer, Raghav R. Poudyal, Philip C. Bevilacqua
Rok vydání:	2021
Předmět:	chemistry.chemical_classification Coacervate RNA Untranslated biology Biochemical Phenomena Biomolecule Ribozyme RNA Proteins RNA Biochemistry Article chemistry biology.protein Biophysics Lipid bilayer Intracellular Macromolecule
Zdroj:	Methods Enzymol Methods in Enzymology ISBN: 9780128211595
ISSN:	1557-7988
Popis:	Liquid-liquid phase separation (LLPS) has been known to drive formation of biomolecular compartments, which can encapsulate RNA and proteins among other cosolutes. Such compartments, which lack lipid membrane, have been implicated in origins of life scenarios as they can easily uptake and concentrate biomolecules, similar to protein-based intracellular condensates. Indeed, chemical interactions that drive LLPS in vitro have also been shown to form similar sub-cellular compartments in vivo. Here we describe methods to prepare compartments formed by complex coacervates, which are formed by LLPS of oppositely-charged polyions, and to probe the structures and functions of RNAs in them. These methods can be adapted to study RNA biochemistry in compartments formed by diverse artificial and biological macromolecules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9d0e5f380a862bacecd0f88019ee193 https://pubmed.ncbi.nlm.nih.gov/33453930 Zobrazit plný text záznamu