In Vitro Formation of Dickeya zeae MS1 Biofilm
| Autor: | Qiyun Yang, Ning Huang, Shen Huifang, Pu Xiaoming, Zhang Jingxin, B. R. Lin, Zhongwen Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Sucrose
Sodium Microbial metabolism chemistry.chemical_element Environment Matrix (biology) Biology Polysaccharide Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Bacterial soft rot chemistry.chemical_compound Microtiter plate Plant Diseases 030304 developmental biology chemistry.chemical_classification 0303 health sciences Microscopy Confocal 030306 microbiology Polysaccharides Bacterial fungi Dickeya chrysanthemi Biofilm food and beverages Musa General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification chemistry Biofilms |
| Zdroj: | Current Microbiology. 76:100-107 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/s00284-018-1593-y |
| Popis: | Bacterial soft rot caused by Dickeya zeae MS1 (Erwinia chrysanthemi) is one of the most devastating banana diseases worldwide. However, knowledge of the development and ecological interactions of D. zeae MS1 biofilm is limited. Here, we visualized the development and architecture of D. zeae MS1 biofilm using confocal laser scanning microscopy, and we evaluated the ability of D. zeae MS1 to form biofilms under different environmental conditions (carbon sources, temperatures, pH levels and mineral elements) using a microtiter plate assay. We found that the development of D. zeae MS1 biofilm could be categorized into four phases and that mature biofilm consisted of a highly organized architecture of both bacterial cells and a self-produced matrix of extracellular polysaccharides. Furthermore, sucrose was the most suitable carbon source for supporting the growth of biofilm cells and that 32 °C and pH 7.0 were the most favorable of the temperatures and pH levels examined. Meanwhile, the addition of Ca2+, Fe2+, K+ and Na+ enhanced the formation of biofilm in minimal medium cultures, whereas 2.5 mM Cu2+ and Mn2+ was inhibitory. A better understanding of biofilm formation under different environmental parameters will improve our knowledge of the growth kinetics of D. zeae MS1 biofilm. |
| Databáze: | OpenAIRE |
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