Purification of high-molecular-weight subfraction from porcine skin inhibiting proliferation of A431 human carcinoma epidermoid cells
| Autor: | V. Ya. Arion, O. V. Belova, E. V. Kovaleva, I. V. Zimina, M. V. Grechikhina, Moskvina Svetlana N, Zh. I. Klyuchnikova, G. V. Lutsenko, T. A. Lukanidina, V. I. Sergienko, G. G. Borisenko |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Ion exchange Tissue Extracts Sus scrofa Antineoplastic Agents General Medicine Biology Chromatography Ion Exchange Molecular biology General Biochemistry Genetics and Molecular Biology In vitro Molecular Weight Electrophoresis Inhibitory Concentration 50 Epidermoid carcinoma Cell Line Tumor medicine Animals Humans Porcine skin Drug Screening Assays Antitumor Cell Proliferation Skin |
| Zdroj: | Bulletin of experimental biology and medicine. 157(3) |
| ISSN: | 1573-8221 |
| Popis: | Subfraction with a molecular weight >250 kDa isolated from porcine skin and inhibiting the proliferation of A431 human carcinoma epidermoid cells was purifi ed by DEAE 32 anion exchange chromatography with NaCl concentration step-gradient. The effects of the initial subfraction and fractions obtained by separation in DEAE 32 on the proliferation of A431 human carcinoma epidermoid cells were studied in vitro in two tests (MTT and fl uorescent test). The more sensitive fl uorescent test showed the highest inhibitory activity of fraction No. 2 released from the column at 0.15 M NaCl. One major protein component and a series of minor protein components were detected in this fraction by vertical PAAG-SDS electrophoresis. |
| Databáze: | OpenAIRE |
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