Toxicological and immunological evaluation of the immunosuppressant 15-deoxyspergualin in BALB/c mice: an in vivo and in vitro study
| Autor: | N Sato, Kouju Kamata, Ken-ichi Amemiya, Eiko Horata, Yoshihiko Masaki, Takaaki Aoyagi, Takehiko Uchiyama, Michihito Okubo |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Interleukin 2
T cell Spleen In Vitro Techniques Pharmacology Guanidines BALB/c Mice T-Lymphocyte Subsets In vivo medicine Splenocyte Animals Antibody-Producing Cells Mice Inbred BALB C Blood Cells biology biology.organism_classification medicine.anatomical_structure Immune System Immunology Toxicity Interleukin-2 Female Liver function Immunosuppressive Agents medicine.drug |
| Zdroj: | Immunopharmacology. 21:99-107 |
| ISSN: | 0162-3109 |
| DOI: | 10.1016/0162-3109(91)90013-o |
| Popis: | The toxicological aspects as well as the immunosuppressive mechanism of 15-deoxyspergualin (DSP) were studied using BALB/c mice. Five-week-old animals were subcutaneously given phosphate-buffered saline (PBS; Group 1) or DSP at 0.5 (Group 2) to 5.0 mg (Group 4) per kg body weight daily for 1 to 3 months. They were sacrificed to obtain blood for hematology and liver function studies. The spleen, taken simultaneously, was histologically examined, the T cell surface markers of splenocytes were flow cytometrically measured, and their interleukin 2 (IL-2) production induced in vitro by Enterotoxin A (Ent A) was assayed using CTLL cells. Additionally, the in vitro effect of DSP on IL-2 generation and plaque forming cell (PFC) production was studied using splenocytes of non-treated mice. During the 3 months of treatment, the body weight slowly increased in Groups 1 and 2, while the body weight of the 2.5-mg DSP mouse group (Group 3) was significantly lower on days 28 and 56 of treatment compared with Group 1 of the same age (P less than 0.05). The administration of DSP at 5.0 mg/kg (Group 4) caused marked reduction in body weight of the animals. They were sacrificed on day 28 because of their worsening general condition. WBC and RBC counts decreased in Group 3 on days 57 and 93, and the platelet number increased on day 57. A liver function test was not affected by DSP treatment except for an elevated SGOT in Group 4.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|