The Effects of the Diterpenes Isolated from the Brazilian Brown AlgaeDictyota pfaffiiandDictyota menstrualisagainst the Herpes Simplex Type-1 Replicative Cycle
| Autor: | Izabel C. P. Paixão, Carlos Frederico Leite Fontes, Diana Negrão Cavalcanti, Jussara P. Barbosa, Valéria Laneuville Teixeira, Thiago Moreno L. Souza, Renato Crespo Pereira, Juliana L. Abrantes |
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| Rok vydání: | 2009 |
| Předmět: |
Pharmaceutical Science
Herpesvirus 1 Human Phaeophyta Virus Replication Antiviral Agents Analytical Chemistry Microbiology Viral Proteins chemistry.chemical_compound Multiplicity of infection Algae Chlorocebus aethiops Drug Discovery Botany Dictyotaceae Animals Vero Cells Pharmacology Dose-Response Relationship Drug biology Plant Extracts Organic Chemistry Herpes Simplex biology.organism_classification In vitro Brown algae Complementary and alternative medicine chemistry Viral replication Vero cell Molecular Medicine Diterpenes Diterpene Brazil Phytotherapy |
| Zdroj: | Planta Medica. 76:339-344 |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | We describe in this paper that the diterpenes 8,10,18-trihydroxy-2,6-dolabelladiene ( 1) and (6 R)-6-hydroxydichotoma-4,14-diene-1,17-dial ( 2), isolated from the marine algae DICTYOTA PFAFFII and D. MENSTRUALIS, respectively, inhibited HSV-1 infection in Vero cells. We initially observed that compounds 1 and 2 inhibited HSV-1 replication in a dose-dependent manner, resulting in EC (50) values of 5.10 and 5.90 microM, respectively, for a multiplicity of infection (MOI) of 5. Moreover, the concentration required to inhibit HSV-1 replication was not cytotoxic, resulting in good selective index (SI) values. Next, we found that compound 1 sustained its anti-herpetic activity even when added to HSV-1-infected cells at 6 h after infection, while compound 2 sustained its activity for up to 3 h after infection, suggesting that these compounds inhibit initial events during HSV-1 replication. We also observed that both compounds were incapable of impairing HSV-1 adsorption and penetration. In addition, the tested molecules could decrease the contents of some HSV-1 early proteins, such as UL-8, RL-1, UL-12, UL-30 and UL-9. Our results suggest that the structures of compounds 1 and 2, Brazilian brown algae diterpenes, might be promising for future antiviral design. |
| Databáze: | OpenAIRE |
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