The cytosolic 5′-nucleotidase cN-II lowers the adaptability to glucose deprivation in human breast cancer cells
| Autor: | Gabriel Bricard, Maria Grazia Tozzi, Léa Payen-Gay, Laure-Estelle Cassagnes, Emeline Cros-Perrial, Charles Dumontet, Lars Petter Jordheim, Jean-Yves Puy, Octavia Cadassou, Isabelle Lefebvre-Tournier |
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| Přispěvatelé: | Centre de Recherche en Cancérologie de Lyon (UNICANCER/CRCL), Centre Léon Bérard [Lyon]-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), University of Pisa - Università di Pisa, Cassagnes, Laure-Estelle, Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
glucose metabolism Cell [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Carbohydrate metabolism Biology 5'-nucleotidase 03 medical and health sciences [SDV.CAN] Life Sciences [q-bio]/Cancer Nucleotidase [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] medicine reactive oxygen species hypoxia Metabolism Transfection 5’-nucleotidase 3. Good health Cytosol 030104 developmental biology medicine.anatomical_structure Oncology Biochemistry Cancer cell Research Paper |
| Zdroj: | Scopus-Elsevier Oncotarget Oncotarget, Impact journals, 2017, ⟨10.18632/oncotarget.18653⟩ Oncotarget, 2017, ⟨10.18632/oncotarget.18653⟩ |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.18653 |
| Popis: | International audience; The cytosolic 5'-nucleotidase cN-II is a highly conserved enzyme implicated in nucleotide metabolism. Based on recent observations suggesting additional roles not directly associated to its enzymatic activity, we studied human cancer cell models with basal or decreased cN-II expression. We developed cancer cells with stable inhibition of cN-II expression by transfection of shRNA-coding plasmids, and studied their biology. We show that human breast cancer cells MDA-MB-231 with decreased cN-II expression better adapt to the disappearance of glucose in growth medium under normoxic conditions than cells with a baseline expression level. This is associated with enhanced in vivo growth and a lower content of ROS in cells cultivated in absence of glucose due to more efficient mechanisms of elimination of ROS. Conversely, cells with low cN-II expression are more sensitive to glucose deprivation in hypoxic conditions. Overall, our results show that cN-II regulates the cellular response to glucose deprivation through a mechanism related to ROS metabolism and defence. |
| Databáze: | OpenAIRE |
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