Vitamin B12 quantification in human milk – Beyond current limitations using liquid chromatography and inductively coupled plasma – Mass spectrometry
| Autor: | Janique Richoz Payot, Paul Sylvain, Stéphane Dubascoux, Marine Nicolas, Esther Campos Giménez |
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| Rok vydání: | 2021 |
| Předmět: |
Population
Sensitivity and Specificity 01 natural sciences Cobalamin Mass Spectrometry Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Humans Sample preparation Vitamin B12 education Inductively coupled plasma mass spectrometry education.field_of_study Chromatography Milk Human 010401 analytical chemistry Microbiological assay Reproducibility of Results 04 agricultural and veterinary sciences General Medicine 040401 food science 0104 chemical sciences Vitamin B 12 Chromatographic separation chemistry Chromatography Liquid Food Science |
| Zdroj: | Food Chemistry. 362:130197 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2021.130197 |
| Popis: | Vitamin B12 plays a key role in human biological functions and is vital in the neurological development of infants. The assessment of the vitamin B12 intake in exclusively breastfed babies depends on the reliability of its determination in milk. In this report, we present a new accurate and robust method for quantification of vitamin B12 in human milk. A highly specific sample preparation is applied, associated with chromatographic separation and detection by ICP-MS. Excellent sensitivity and accuracy are reported, with recovery values well within acceptability limits (80-120 %), within- and between-day variability are lower than 10 % and 15 % respectively. Strong correlation with a microbiological assay was observed (r2=0.9) within the validation range (40-1000 pmol/L corresponding to 54 to 1355 ng/L). The method can be used to routinely monitor vitamin B12 in clinical or population observational studies, determine infant’s intake or assess efficacy of mother’s supplementation. |
| Databáze: | OpenAIRE |
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