The use of Silver-stained 'Comets' to Visualize DNA Damage and Repair in Normal and Xeroderma pigmentosum Fibroblasts After Exposure to Simulated Solar Radiation
| Autor: | Wayne Gorman, M Rouabhia, Yvon Deslauriers, P Reinhardt-Poulin, S Cabat, J. R. N. McLean |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
animal structures Xeroderma pigmentosum DNA damage Comet food and beverages General Medicine Biology Radiation medicine.disease complex mixtures Molecular biology Biochemistry Silver stain chemistry.chemical_compound chemistry In vivo medicine sense organs Irradiation biological phenomena cell phenomena and immunity Physical and Theoretical Chemistry DNA |
| Zdroj: | Photochemistry and Photobiology. 71:422-425 |
| ISSN: | 0031-8655 |
| DOI: | 10.1562/0031-8655(2000)0710422tuossc2.0.co2 |
| Popis: | The alkaline and neutral comet assays have been widely used to assess DNA damage and repair in individual cells after in vivo or in vitro exposure to chemical or physical genotoxins. Cells processed under neutral conditions generate comets primarily from DNA double strand breaks, whereas under alkaline conditions, comets arise from DNA single and double strand breaks and alkali-labile lesions. A modified version of the alkaline comet assay, as described here, used silver stain to visualize the comets and a ™Gelbond base to facilitate the manipulation and processing of samples. To demonstrate how these modifications improve the assay, fibroblasts derived from both normal and Xeroderma pigmentosum (Xp) individuals were exposed to simulated solar radiation and the resulting DNA damage and repair evaluated and compared with results from the relevant literature. Comets from normal fibroblasts reached their maximum length at about an hour after irradiation. Dose-dependent increases in comet length were... |
| Databáze: | OpenAIRE |
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