The Role of Cardiac N-Methyl-D-Aspartate Receptors in Heart Conditioning—Effects on Heart Function and Oxidative Stress
| Autor: | Sergey Bolevich, Jovana Jeremic, Jovana Bradic, Vladimir Jakovljevic, Katarina Radonjic, Dragan Djuric, Marijana Andjic, Ivan Srejovic, Natalia Govoruskina, Nevena Draginic, Aleksandra Stojanovic, Israpil Alisultanovich Omarov, Isidora Milosavljevic, Vladimir Zivkovic |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Ischemia lcsh:QR1-502 Myocardial Reperfusion Injury glutamate heart Pharmacology medicine.disease_cause Receptors N-Methyl-D-Aspartate Biochemistry Article lcsh:Microbiology 03 medical and health sciences 0302 clinical medicine conditioning cardiodynamics medicine Animals oxidative stress Rats Wistar Receptor Molecular Biology Chemistry Glutamate receptor Memantine medicine.disease 3. Good health 030104 developmental biology nervous system Glycine NMDA receptor N-methyl-D-aspartate receptor Reperfusion injury 030217 neurology & neurosurgery Oxidative stress medicine.drug |
| Zdroj: | Biomolecules, Vol 10, Iss 1065, p 1065 (2020) Biomolecules Volume 10 Issue 7 |
| Popis: | As well as the most known role of N-methyl-D-aspartate receptors (NMDARs) in the nervous system, there is a plethora of evidence that NMDARs are also present in the cardiovascular system where they participate in various physiological processes, as well as pathological conditions. The aim of this study was to assess the effects of preconditioning and postconditioning of isolated rat heart with NMDAR agonists and antagonists on heart function and release of oxidative stress biomarkers. The hearts of male Wistar albino rats were subjected to global ischemia for 20 min, followed by 30 min of reperfusion, using the Langendorff technique, and cardiodynamic parameters were determined during the subsequent preconditioning with the NMDAR agonists glutamate (100 µ mol/L) and (RS)-(Tetrazol-5-yl)glycine (5 &mu mol/L) and the NMDAR antagonists memantine (100 &mu mol/L) and MK-801 (30 &mu mol/L). In the postconditioning group, the hearts were perfused with the same dose of drugs during the first 3 min of reperfusion. The oxidative stress biomarkers were determined spectrophotometrically in samples of coronary venous effluent. The NMDAR antagonists, especially MK-801, applied in postconditioning had a marked antioxidative effect with a most pronounced protective effect. The results from this study suggest that NMDARs could be a potential therapeutic target in the prevention and treatment of ischemic and reperfusion injury of the heart. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|