The unfolded protein response in a dolichyl phosphate mannose-deficient Chinese hamster ovary cell line points out the key role of a demannosylation step in the quality-control mechanism of N-glycoproteins
| Autor: | Foulquier, Francois, Harduin-Lepers, Anne, Duvet, Sandrine, Marchal, Ingrid, Marie MIR, Anne, Delannoy, Philippe, Chirat, Frédéric, Cacan, René |
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| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Université Lille Nord de France (COMUE), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Transcription Genetic MESH: Glycoproteins Oligosaccharides MESH: Cricetinae [SDV.CAN]Life Sciences [q-bio]/Cancer CHO Cells [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy MESH: Actins Biochemistry Cell Line 03 medical and health sciences 0302 clinical medicine MESH: Dolichol Monophosphate Mannose MESH: CHO Cells Cricetinae [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Animals MESH: Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Glycoproteins 0303 health sciences MESH: Kinetics [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE] MESH: Transcription Genetic [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] Cell Biology [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] Actins [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] MESH: Cell Line carbohydrates (lipids) Kinetics MESH: Oligosaccharides 030217 neurology & neurosurgery Dolichol Monophosphate Mannose Research Article |
| Zdroj: | Biochemical Journal Biochemical Journal, Portland Press, 2002, 362 (Pt 2), pp.491-8 Biochemical Journal, Portland Press, 2002, 362 (2), pp.491. ⟨10.1042/0264-6021:3620491⟩ Biochemical Journal, 2002, 362 (Pt 2), pp.491-8 Biochemical Journal, 2002, 362 (2), pp.491. ⟨10.1042/0264-6021:3620491⟩ |
| ISSN: | 0264-6021 1470-8728 |
| DOI: | 10.1042/0264-6021:3620491⟩ |
| Popis: | International audience; The CHO (Chinese hamster ovary) glycosylation mutant cell line, B3F7, transfers the truncated glycan Glc(3)Man(5)GlcNAc(2) on to nascent proteins. After deglucosylation, the resulting Man(5)GlcNAc(2) glycan is subjected to two reciprocal enzymic processes: the action of an endoplasmic-reticulum (ER) kifunensine-sensitive alpha1,2-mannosidase activity to yield a Man(4)GlcNAc(2) glycan, and the reglucosylation involved in the quality-control system which ensures that only correctly folded glycoproteins leave the ER. We show that the recombinant secreted alkaline phosphatase (SeAP) produced in stably transfected B3F7 cells, is co-immunoprecipitated with the GRP78 (glucose-regulated protein 78), a protein marker of the unfolded protein response (UPR). The level of GRP78 transcription has been evaluated by reverse transcription-PCR (RT-PCR) and we demonstrate that B3F7 cells present a constitutively higher level of UPR in the absence of inductors, compared with Pro(-5) cells. Interestingly, a decrease was observed in the UPR and an increase in SeAP secretion in the kifunensine-treated B3F7 cells. Altogether, these data highlight the relationships between the glycan structure, the quality control system and the UPR. Moreover, they support the idea that a specific demannosylation step is a key event of the glycoprotein quality control in B3F7 cells. |
| Databáze: | OpenAIRE |
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