Comparison of antinuclear antibody testing methods: immunofluorescence assay versus enzyme immunoassay
| Autor: | R A Gniewek, T M McHugh, Joan F. Hilton, Mayumi Nakagawa, D P Stites |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Microbiology (medical)
CREST Syndrome Pathology medicine.medical_specialty Anti-nuclear antibody Discoid lupus erythematosus Clinical Biochemistry Immunology Fluorescent Antibody Technique Reference range Sensitivity and Specificity Immunoenzyme Techniques Mixed connective tissue disease Predictive Value of Tests Reference Values immune system diseases medicine Humans Immunology and Allergy Connective Tissue Diseases skin and connective tissue diseases Retrospective Studies Systemic lupus erythematosus business.industry Sclerodactyly Dermatomyositis medicine.disease Evaluation Studies as Topic Antibodies Antinuclear medicine.symptom business Research Article |
| Zdroj: | Clinical Diagnostic Laboratory Immunology. 4:185-188 |
| ISSN: | 1098-6588 1071-412X |
| DOI: | 10.1128/cdli.4.2.185-188.1997 |
| Popis: | Performances of anti-nuclear antibody testing by immunofluorescence assay (ANA-IFA) and enzyme immunoassay (ANA-EIA) were compared in relation to patient diagnosis. A total of 467 patient serum samples were tested by ANA-IFA (Kallestad; Sanofi) and ANA-EIA (RADIAS; Bio-Rad), and their age, sex, diagnosis, disease status, and medications were obtained through chart review. Reference ranges were established by testing 98 healthy blood donor samples. Eighty-six samples came from patients with diffuse connective tissue diseases, including systemic lupus erythematosus, discoid lupus erythematosus, or drug-induced lupus (n = 71); systemic sclerosis, CREST syndrome (calcinosis, Raynaud's phenomenon, esophageal motility abnormalities, sclerodactyly, and telangiectasia), or Raynaud's syndrome (n = 8); Sjögren's syndrome (n = 5); mixed connective tissue disease (n = 5); and polymyositis or dermatomyositis (n = 3). The sensitivity, specificity, positive predictive value, and negative predictive value for ANA-IFA were 87.2, 48.0, 29.1, and 93.9%, respectively, for the reference range of < 1:160. For ANA-EIA, they were 90.7, 60.2, 35.8, and 96.4%, respectively, for the reference range of < 0.9. ANA-EIA offers equivalent sensitivity and higher specificity compared to ANA-IFA. |
| Databáze: | OpenAIRE |
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