Serum Response Factor Protects Retinal Ganglion Cells Against High-Glucose Damage
| Autor: | Liang Wang, Ying Tian, Junhong Zhao, Qiang Ma, Hongbing Zhang, Houcheng Liang, Yan Cao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Retinal Ganglion Cells
0301 basic medicine Serum Response Factor genetic structures NF-E2-Related Factor 2 Apoptosis Biology Retinal ganglion Rats Sprague-Dawley 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Western blot Downregulation and upregulation Serum response factor medicine Animals Transcription factor Cells Cultured Gene knockdown medicine.diagnostic_test General Medicine Molecular biology eye diseases Rats Up-Regulation Glucose 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis embryonic structures cardiovascular system sense organs Proto-Oncogene Proteins c-fos |
| Zdroj: | Journal of Molecular Neuroscience. 59:232-240 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-015-0708-1 |
| Popis: | Serum response factor (SRF), which encodes the MADS-box family of related proteins, is a common transcription factor related to the expression of genes associated with cell survival. However, SRF's role in retinal ganglion cells (RGCs) after high-glucose injury remains unclear. In this study, we investigate the protective role of SRF after high-glucose injury and its underlying mechanism. The in vitro RGC model subjected to high glucose was established by employing a 50 mmol/L glucose culture environment. As detected by real-time quantitative PCR and Western blot, SRF was significantly upregulated in RGCs treated with high glucose. Overexpression of SRF significantly promoted survival among RGCs exposed to high glucose and inhibited RGC apoptosis. Knockdown of SRF exerted an inverse effect. Moreover, SRF upregulation enhanced expression of an antioxidant protein, nuclear factor erythroid 2-related factor (Nrf2), via control of the Fos-related antigen 1 (Fra-1). SRF upregulation also affected RGC survival after high-glucose treatment. Our findings showed that overexpression of SRF promoted survival of RGCs after high-glucose injury by regulating Fra-1 and Nrf2. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink