Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35
| Autor: | Elina Alaterre, Adrien Nivoliez, Philippe Langella, Philippe Veisseire, Luis G. Bermúdez-Humarán, Stéphanie Bornes, Magaly Angénieux, Christiane Forestier, N. Kondjoyan, Laurence Nakusi, Sylvie Miquel, Caroline Dausset, Erwan Engel |
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| Přispěvatelé: | biose Industrie, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Unité Mixte de Recherche sur le Fromage (UMRF), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Qualité des Produits Animaux (QuaPA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Rok vydání: | 2020 |
| Předmět: |
composé soufré
0301 basic medicine Antifungal Agents Science 030106 microbiology Cell Thiosulfates Excipient [SDV.BC]Life Sciences [q-bio]/Cellular Biology Sodium thiosulfate Acetates In Vitro Techniques Article Microbiology 03 medical and health sciences chemistry.chemical_compound Lactobacillus rhamnosus Candida albicans medicine Humans Candidiasis Vulvovaginal Multidisciplinary biology Sulfur Compounds Bacteria Lacticaseibacillus rhamnosus Antimicrobials Microbiota Probiotics Fungi Potentiator biology.organism_classification In vitro Yeast Corpus albicans Coculture Techniques Microscopy Electron 030104 developmental biology medicine.anatomical_structure chemistry Odorants Vagina Medicine Female medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.17074. ⟨10.1038/s41598-020-74027-7⟩ Scientific Reports, 2020, 10 (1), pp.17074. ⟨10.1038/s41598-020-74027-7⟩ Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-74027-7⟩ |
| Popis: | GYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida activity of L. rhamnosus Lcr35, with a complete loss of viability of the yeast after 48 h of coincubation. Sodium thiosulfate (STS), one excipient of the product, was used as a potentiator of the anti-Candida spp. activity of Lactobacilli. This contact-independent phenomenon induced fungal cell disturbances, as observed by electron microscopy observations. Nonverbal sensory experiments showed clear odor dissimilarities between cocultures of L. rhamnosus Lcr35 and C. albicans in the presence and absence of STS, suggesting an impact of odor-active metabolites. A volatolomic approach allowed the identification of six odor-active compounds, including one sulfur compound that was identified as S-methyl thioacetate (MTA). MTA was associated with the antifungal effect of Lcr35, and its functional link was established in vitro. We show for the first time that the LBP GYNOPHILUS, which is a highly active product in the reduction of vulvovaginal candidiasis, requires the presence of a sulfur compound to fully achieve its antifungal effect. |
| Databáze: | OpenAIRE |
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