A GC cluster repeat is a hotspot for mit- macro-deletions in yeast mitochondrial DNA
| Autor: | Rudolf J. Schweyen, Sang Ho Kim, Elke Pratje, Helmut Bruckner, Georg F. Weiller |
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| Rok vydání: | 1991 |
| Předmět: |
Mitochondrial DNA
Mutant Genes Fungal Molecular Sequence Data Restriction Mapping Saccharomyces cerevisiae Biology DNA Mitochondrial Electron Transport Complex IV Cytosine nucleotide Restriction map Gene mapping Genetics Cloning Molecular DNA Fungal Molecular Biology Gene Repetitive Sequences Nucleic Acid Recombination Genetic Base Composition Base Sequence Intron DNA Restriction Enzymes Molecular biology Introns Multigene Family Mobile genetic elements Chromosome Deletion |
| Zdroj: | Moleculargeneral genetics : MGG. 226(1-2) |
| ISSN: | 0026-8925 |
| Popis: | In a random collection of mit− mutations of the yeast strain 777-3A we find that deletions are exceptionally frequent in the OXI3 gene, a large mosaic gene coding for subunit I of cytochrome oxidase. About 10% of all oxi3 − mutants carry the same macro-deletion, del-A, extending from the 5′ non-translated leader of OXI3 to intron 5b of this gene. Determination of the respective wild-type sequences and of the del-A junction sequence revealed that the end-points of the deletion are in two GC clusters with 31 by sequence identity which are located at a distance of 11.3 kb. We speculate that not only the sequence identity of the two GC clusters but also the palindromic structure of these putatively mobile elements of yeast mitochondrial DNA (mtDNA) plays a role in deletion formation. |
| Databáze: | OpenAIRE |
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