FGF-2 and VEGF functionalization of starPEG–heparin hydrogels to modulate biomolecular and physical cues of angiogenesis
| Autor: | Petra B. Welzel, Carsten Werner, Silvana Prokoph, Uwe Freudenberg, Andrea Zieris, Milauscha Grimmer, Kandice R. Levental |
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| Rok vydání: | 2010 |
| Předmět: |
Vascular Endothelial Growth Factor A
Umbilical Veins Materials science Angiogenesis medicine.medical_treatment Basic fibroblast growth factor Biophysics Neovascularization Physiologic Biocompatible Materials Bioengineering Fibroblast growth factor Fluorescence Umbilical vein Polyethylene Glycols Biomaterials chemistry.chemical_compound Tissue engineering medicine Humans Cells Cultured Tissue Scaffolds Heparin Rhodamines Growth factor Endothelial Cells Hydrogels Vascular endothelial growth factor Immobilized Proteins chemistry Mechanics of Materials Self-healing hydrogels Ceramics and Composites Fibroblast Growth Factor 2 Biomedical engineering |
| Zdroj: | Biomaterials. 31:7985-7994 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2010.07.021 |
| Popis: | Tissue engineering therapies require biomaterials capable of encouraging an angiogenic response. To dissect the influence of different pro-angiogenic stimuli a set of starPEG-heparin hydrogels with varied physicochemical properties was used as a highly efficient reservoir and tunable delivery system for basic fibroblast growth factor (FGF-2) and vascular endothelial growth factor (VEGF). The engineered gel materials could be precisely tailored by decoupling the biomolecular functionalization from the variation of the viscoelastic matrix characteristics. Culture experiments with human umbilical vein endothelial cells (HUVECs) revealed the interplay of growth factor presentation, adhesive characteristics and elasticity of the gel matrices in triggering differential cellular behavior which allowed identifying effective pro-angiogenic conditions. |
| Databáze: | OpenAIRE |
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