Perinatal rat lung catalase gene expression: influence of corticosteroid and hyperoxia
| Autor: | D. Massaro, J. Iqbal, Linda B. Clerch |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aging medicine.medical_specialty Physiology medicine.drug_class Gestational Age Dexamethasone Gene Expression Regulation Enzymologic Physiology (medical) Internal medicine Gene expression medicine Animals RNA Messenger Lung chemistry.chemical_classification Hyperoxia Messenger RNA biology Cell Biology Blotting Northern Catalase Rats Oxygen Endocrinology Enzyme chemistry biology.protein RNA Gestation Corticosteroid medicine.symptom medicine.drug |
| Zdroj: | American Journal of Physiology-Lung Cellular and Molecular Physiology. 260:L428-L433 |
| ISSN: | 1522-1504 1040-0605 |
| DOI: | 10.1152/ajplung.1991.260.6.l428 |
| Popis: | Dexamethasone accelerates the late gestational rise in rat lung catalase activity; neonatal hyperoxia elevates rat lung catalase activity. We studied the regulation of catalase gene expression in these instances. Catalase mRNA/mg DNA increased to gestation day 22 and then fell to the concentration in adult lungs. The rate of transcription of catalase mRNA was higher on gestation day 22 than gestation day 19, whereas the half-life of catalase mRNA (approximately 7 h) was the same on both days. Dexamethasone given 48 and 24 h before expected birth (gestation 22 days) increased catalase mRNA concentration at days 20 and 22 without a change in catalase mRNA stability. Early postnatal hyperoxia (greater than 95% O2, 72 h) elevated catalase mRNA/mg DNA and doubled its half-life without changing its rate of transcription. We conclude the normal late gestational elevation of catalase activity and the increase of activity during prenatal dexamethasone treatment are regulated at the level of gene transcription. By contrast, the elevation of catalase activity during neonatal hyperoxia is mediated posttranscriptionally by increased catalase mRNA stability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|