Recovery after PILP remineralization of dentin lesions created with two cariogenic acids
| Autor: | Yung-Ching Chien, Stefan Habelitz, Grayson W. Marshall, Kuniko Saeki, Alexander F. Chin, Sally J. Marshall, Nonomura G, Laurie B. Gower |
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| Rok vydání: | 2017 |
| Předmět: |
Molar
Polymers Dentistry 02 engineering and technology Demineralization Acetates Microscopy Atomic Force Atomic force microscopy 0302 clinical medicine Dentin Tooth Demineralization Shrinkage Microscopy Chemistry PILP mineralization Dentin caries models Atomic Force General Medicine 021001 nanoscience & nanotechnology medicine.anatomical_structure medicine.symptom 0210 nano-technology Remineralization Surface Properties In Vitro Techniques Electron Article Lesion 03 medical and health sciences Recovery rate Microscopy Electron Transmission Elastic Modulus Nanomechanical property medicine Humans Transmission Lactic Acid General Dentistry Third Remineralisation Chromatography business.industry 030206 dentistry Cell Biology Otorhinolaryngology Tooth Remineralization Molar Third business Peptides Zoology |
| Zdroj: | Saeki, K; Chien, Y-C; Nonomura, G; Chin, AF; Habelitz, S; Gower, LB; et al.(2017). Recovery after PILP remineralization of dentin lesions created with two cariogenic acids. ARCHIVES OF ORAL BIOLOGY, 82, 194-202. doi: 10.1016/j.archoralbio.2017.06.006. UCSF: Retrieved from: http://www.escholarship.org/uc/item/98m8h3w3 |
| DOI: | 10.1016/j.archoralbio.2017.06.006. |
| Popis: | Objectives Acetate and lactate are important cariogenic acids produced by oral bacteria. They produced different residual dentin structures in artificial lesions of similar depth. We evaluated if such lesions responded in the same way to a polymer-induced-liquid-precursor (PILP) remineralization. Design Dentin blocks obtained from human third molars, divided into 6 groups (n = 3). Blocks were demineralized with acetate (66 h) or lactate (168 h) buffer at pH 5.0 to create 140 μm target lesion depths. A-DEM and L-DEM groups received no remineralization. Other groups were remineralized for 14 days. 100 μg/mL polyaspartate was added into the remineralizing buffer for A-PIL and L-PIL, whereas A-CAP and L-CAP were treated with the same solution but without polyaspartate. Cross-sectioned blocks were examined for shrinkage and AFM-topography. Line profiles of reduced elastic modulus (E r ) were obtained by AFM-based nanoindentation across the lesion. Ultrastructures were examined with TEM. Results A-PIL and L-PIL recovered in shrinkage to the original height of the dentin and it appeared normal with tubules, with increases in E r at both outer flat and inner sloped zones. At the sloped zone, acetate lesions lost more E r but recovery rate after PILP was not statistically different from lactate lesions. A-CAP and L-CAP showed surface precipitates, significantly less recovery in shrinkage or E r as compared to PILP groups. TEM-ultrastructure of PILP groups showed similar structural and mineral components in the sloped zone for lesions produced by either acid. Conclusions The PILP process provided significant recovery of both structure and mechanical properties for artificial lesions produced with acetate or lactate. |
| Databáze: | OpenAIRE |
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