Effect of antibiotics and NSAIDs on cyclooxygenase-2 in the enamel mineralization
| Autor: | Ascensión Vicente, Antonio José Ortiz Ruiz, Amparo Pérez Silva, Clara Serna Muñoz, Maria Teresa Castells, Francisco Solano |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Molar medicine.medical_specialty lcsh:Medicine Article Mice 03 medical and health sciences 0302 clinical medicine stomatognathic system Internal medicine medicine Animals lcsh:Science Dental Enamel Multidisciplinary biology Chemistry lcsh:R Anti-Inflammatory Agents Non-Steroidal Enamel organ 030206 dentistry Amelogenesis Amoxicillin Anti-Bacterial Agents Acetaminophen stomatognathic diseases Enamel mineralization 030104 developmental biology Endocrinology Cyclooxygenase 2 biology.protein Celecoxib lcsh:Q Cyclooxygenase Tooth Calcification medicine.drug |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-7 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-22607-z |
| Popis: | The objective of this study was to determine whether the use of the most commonly prescribed antibiotics and non-steroidal anti-inflammatory drugs in childhood could disturb enamel mineralization. Forty-two Swiss mice were divided into seven groups: controls; amoxicillin; amoxicillin/clavulanate; erythromycin; acetaminophen; ibuprofen and celecoxib, to inhibit cyclooxygenase 2 (COX2). SEM-EDX analysis was conducted on all cusps of the third molars. Calcium (Ca), phosphorus (P), aluminum, potassium, sodium, magnesium and chlorine were quantified. The stoichiometric Ca/P molar ratios were calculated. Immunohistochemical quantification of COX2 in incisors was carried out by image analysis using COX2-specific immunostaining. Groups treated with antibiotics showed no significant differences in the content of the chemical elements. Only acetaminophen and celecoxib showed a significant decrease in Ca and P compared with the control samples. Ca/P ratios showed no difference. Groups treated with amoxicillin, amoxicillin/clavulanate, erythromycin and acetaminophen showed significantly lower amounts of immunoreactive COX2 at the enamel organ maturation stage of the mouse incisors. Our results suggest that COX2 is involved in the maturation stage of the enamel organ and that its inhibition would appear to alter amelogenesis, producing hypomineralization. |
| Databáze: | OpenAIRE |
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