Evaluation of mRNA by Q-RTPCR and Protein Expression by AQUA of the M2 Subunit of Ribonucleotide Reductase (RRM2) in Human Tumors
| Autor: | Kyle D. Holen, Dona Alberti, Jens C. Eickhoff, Percy Ivy, Steven Attia, Anne M. Traynor, William R. Schelman, Wei Huang, Jill M. Kolesar, Kristine Hahn, George Wilding |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Male
Thiosemicarbazones Cancer Research Ribonucleotide Esophageal Neoplasms Ribonucleoside Diphosphate Reductase Pyridines Protein subunit Biology Reductase Toxicology Polymerase Chain Reaction Article Gene Expression Regulation Enzymologic chemistry.chemical_compound Clinical Trials Phase II as Topic Stomach Neoplasms Gene expression Humans Pharmacology (medical) RNA Messenger Pharmacology Messenger RNA Clinical Trials Phase I as Topic 3-Aminopyridine-2-carboxaldehyde thiosemicarbazone Prostatic Neoplasms Molecular biology Gene Expression Regulation Neoplastic Protein Subunits Real-time polymerase chain reaction Ribonucleotide reductase Oncology chemistry |
| Popis: | The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP.Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA).Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- 0.94 SD, vs 0.41 +/- 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- 1.49 SD, vs 0.29 +/- 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12).RRM2 gene and protein expression varies by tumor type. |
| Databáze: | OpenAIRE |
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