Vascular movement of beet necrotic yellow vein virus in Beta macrocarpa is probably dependent on an RNA 3 sequence domain rather than a gene product
| Autor: | G. Jonard, K. E. Richards, Tetsuo Tamada, H. Guilley, Emmanuelle Lauber |
|---|---|
| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 1998 |
| Předmět: |
0106 biological sciences
Transcription Genetic [SDV]Life Sciences [q-bio] viruses Mutant Chenopodiaceae Virus Replication Polymerase Chain Reaction 01 natural sciences Benyvirus Virus Plant Viruses Open Reading Frames Viral Proteins 03 medical and health sciences Virology RNA Viruses Beet necrotic yellow vein virus Gene ComputingMilieux_MISCELLANEOUS Sequence Deletion 030304 developmental biology Subgenomic mRNA 0303 health sciences biology Point mutation RNA biology.organism_classification Plant Leaves Mutagenesis Site-Directed RNA Viral 010606 plant biology & botany |
| Zdroj: | Journal of General Virology Journal of General Virology, Microbiology Society, 1998, 79 (2), pp.385-393. ⟨10.1099/0022-1317-79-2-385⟩ |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-79-2-385 |
| Popis: | RNAs 1 and 2 of beet necrotic yellow vein virus (BNYVV) carry the functions enabling viral RNA replication, cell-to-cell movement, virus assembly and vascular movement of the virus in the systemic host Spinacea oleracea. In Beta macrocarpa, on the other hand, BNYVV RNA 3 is required for vascular movement. Replication-competent RNA 3 transcripts carrying various point mutations and deletions were coinnoculated with RNAs 1 and 2 to young leaves of B. macrocarpa and the ability of the virus to multiply on the inoculated leaves and to invade the plant systemically was examined. None of the RNA 3 mutants tested interfered with virus multiplication in the inoculated leaves. Point mutations designed to specifically block or truncate translation of the ORFs of the two known RNA 3 gene products, P25 and N, did not interfere with vascular movement. Vascular movement was not inhibited by deletions eliminating the short 5'-proximal ORF on RNA 3 (ORF A) or by point mutations blocking putative translation of the short 5'-proximal ORF (ORF S) on RNA 3sub, a subgenomic RNA derived from RNA 3. On the other hand, deletions in a 'core region' encompassing nucleotides 1033-1257 of RNA 3 completely blocked vascular movement of the virus while removal of sequences flanking the core region lowered its efficiency. The observations suggest that some feature of the RNA 3 sequence rather than an RNA-3 coded protein is important for vascular movement of BNYVV in B. macrocarpa. |
| Databáze: | OpenAIRE |
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