Repeat Sequences in Block 2 of Plasmodium falciparum Merozoite Surface Protein 1 Are Targets of Antibodies Associated with Protection from Malaria
| Autor: | Polley, S D, Tetteh, K K A, Cavanagh, D R, Pearce, R J, Lloyd, J M, Bojang, K A, Okenu, D M N, Greenwood, B M, McBride, J S, Conway, D J, Cavanagh, David |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Antibodies
Protozoan ANTIGEN GENE VACCINE T-CELL Epitope Immunoglobulin G law.invention Mice Antibody Specificity law INFECTION Malaria Falciparum Child Merozoite Surface Protein 1 FRAGMENT biology Vaccination Infectious Diseases Child Preschool Recombinant DNA Antibody Adult Molecular Sequence Data Plasmodium falciparum IMMUNE Immunology Antigens Protozoan Microbiology AOTUS MONKEYS Antigen Malaria Vaccines parasitic diseases medicine Animals Humans Amino Acid Sequence PARASITE Antigen Gene Repetitive Sequences Nucleic Acid EFFICACY medicine.disease biology.organism_classification Virology Mice Inbred CBA biology.protein Parasitology Fungal and Parasitic Infections Malaria RESPONSES |
| Zdroj: | Polley, S D, Tetteh, K K A, Cavanagh, D R, Pearce, R J, Lloyd, J M, Bojang, K A, Okenu, D M N, Greenwood, B M, McBride, J S, Conway, D J & Cavanagh, D 2003, ' Repeat sequences in block 2 of Plasmodium falciparum merozoite surface protein 1 are targets of antibodies associated with protection from malaria ', Infection and Immunity, vol. 71, no. 4, pp. 1833-1842 . https://doi.org/10.1128/IAI.71.4.1833-1842.2003 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.71.4.1833-1842.2003 |
| Popis: | Human antibodies to the block 2 region of Plasmodium falciparum merozoite surface protein 1 (MSP1) are associated with a reduced prospective risk of clinical malaria. Block 2 is highly polymorphic, but all known alleles can be grouped into three major types. Two of these types (the K1-like and MAD20-like types) contain type-specific sequences (found in all alleles of a particular type) that flank polymorphic tripeptide repeats. These repeats contain both type-specific and subtype-specific sequences. To evaluate the antibody recognition of these parts of block 2, a new panel of six recombinant proteins was used (fused type-specific flanking sequences and two representative repeat sequences for each of the K1-like and MAD20-like types separately). Extensive testing of these antigens and full-length block 2 antigens showed that human serum immunoglobulin G antibodies induced by infection can recognize (i) type-specific epitopes in the repeats, (ii) subtype-specific epitopes in the repeats, or (iii) type-specific epitopes in flanking sequences. A large prospective study in The Gambia showed that antibodies to the repeats are strongly associated with protection from clinical malaria. The results are important for design of a vaccine to induce protective antibodies, and they address hypotheses about repeat sequences in malaria antigens. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|