Molecular cloning of WHI2, a gene involved in the regulation of cell proliferation in Saccharomyces cerevisiae
| Autor: | D. J. Saul, Peter E. Sudbery |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Genetics
biology Genetic Linkage Saccharomyces cerevisiae Structural gene Genes Fungal DNA Recombinant Chromosome Mapping Locus (genetics) Molecular cloning biology.organism_classification Microbiology Restriction map Plasmid Transformation Genetic Genes Mutation Autoradiography Genomic library Cloning Molecular DNA Fungal Gene Cell Division Plasmids |
| Zdroj: | Journal of general microbiology. 131(7) |
| ISSN: | 0022-1287 |
| Popis: | Summary: The WH12 gene of Saccharomyces cerevisiae plays a key role in co-ordinating cell proliferation and nutrient availability. A 2·6 kb yeast DNA sequence has been cloned which fully suppresses the whi2 mutation. Integration of this sequence to demonstrate that the structural gene itself had been cloned proved difficult. Integration occurred only rarely and only at the LEU2 locus which was also present on the integrating plasmid. To circumvent these difficulties an adjacent sequence, present on the original isolate from the gene library, was subcloned onto the integrating vector YIp5, after which directed integration proved straightforward. The integrated sequence was closely linked to WHI2, confirming that the structural gene had been cloned. A chromosomal restriction map of the WHI2 region is presented; no gross changes were observed in the region as cells entered stationary phase. |
| Databáze: | OpenAIRE |
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