Modeling cell-substrate de-adhesion dynamics under fluid shear
| Autor: | Gautam I. Menon, Garima Rani, Pramod A. Pullarkat, Renu Maan |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Rheometer Biophysics Total internal reflection microscopy 02 engineering and technology Models Biological 3T3 cells law.invention Stress (mechanics) Focal adhesion 03 medical and health sciences Mice Structural Biology Confocal microscopy law medicine Shear strength Shear stress Cell Adhesion Animals Humans Cell adhesion Molecular Biology Shearing (physics) HEK 293 cells Dynamics (mechanics) Cell Biology Adhesion Mechanics 3T3 Cells 021001 nanoscience & nanotechnology Biomechanical Phenomena 030104 developmental biology medicine.anatomical_structure HEK293 Cells Shear (geology) Stress Mechanical 0210 nano-technology Shear Strength |
| Zdroj: | Physical biology. 15(4) |
| ISSN: | 1478-3975 |
| Popis: | Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as for the decay in the fraction of detached cells at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of ~ 500 pN associated to a single focal contact, and characteristic time-scales of 190 ≤ τ ≤ 350s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis. |
| Databáze: | OpenAIRE |
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