Vitronectin Protects Alveolar Macrophages from Silica Toxicity
| Autor: | Rajamouli Pasula, Nicholas A. Doyle, William J. Martin, Paul Wisniowski, Min Wu, Robert W. Spech |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Free Radicals Thiobarbituric acid Blotting Western Critical Care and Intensive Care Medicine Rats Sprague-Dawley chemistry.chemical_compound In vivo Silicosis Macrophages Alveolar medicine Animals Vitronectin Cells Cultured Respiratory Burst medicine.diagnostic_test biology business.industry respiratory system Silicon Dioxide medicine.disease Immunohistochemistry Molecular biology In vitro Rats Respiratory burst body regions Bronchoalveolar lavage medicine.anatomical_structure chemistry biology.protein Pulmonary alveolus business Bronchoalveolar Lavage Fluid |
| Zdroj: | American Journal of Respiratory and Critical Care Medicine. 162:733-739 |
| ISSN: | 1535-4970 1073-449X |
| Popis: | Silicosis is an interstitial lung disease caused by the inhalation of crystalline silicon dioxide. Current concepts suggest that a crucial step in the development of silicosis is silica-induced injury of alveolar macrophages (AM). The adhesive protein vitronectin is a natural constituent of the lung, in which its function is largely unexplored. This study investigated a possible role for vitronectin in protecting AM from silica exposure. In this study, the concentration of vitronectin was shown to be increased in the bronchoalveolar lavage fluid of silica-treated rats. Vitronectin affinity for silica was shown both in vitro and in vivo by immunostaining. Vitronectin reduced silica-induced injury to cultured AM as determined with the (51)Cr release assay. Vitronectin reduced silica-induced free radical production as determined with a cell-free thiobarbituric acid assay. Additionally, vitronectin reduced the silica-induced respiratory burst in AM as determined with chemiluminescence. This study suggests that vitronectin may protect AM during the initial exposure to silica. |
| Databáze: | OpenAIRE |
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