Progenitor endothelial cells on vascular grafts: an ultrastructural study
| Autor: | Sakae Ogata, Hiroshi Yamamoto, Hiroo Iwata, Akihiro Fukui, Manabu Maeda, Yuji Inada, Toshifumi Nakamura, S. Tamai, Yoshito Ikada, Kouko Tatsumi-Nagano, Satomi Haga |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
Pathology medicine.medical_specialty Endothelium Antimetabolites Cell Biomedical Engineering CD34 progenitor endothelial cells Biology Biomaterials Vasculogenesis Cell Movement medicine Animals graft healing Progenitor cell Polytetrafluoroethylene Progenitor Microscopy Confocal vascular graft Stem Cells Immunohistochemistry Rats Inbred F344 Blood Vessel Prosthesis Rats Endothelial stem cell medicine.anatomical_structure Bromodeoxyuridine Microscopy Electron Scanning Ultrastructure Blood Vessels Endothelium Vascular PTFE Flk-1 Cell Division |
| Zdroj: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. 51(1):55-60 |
| ISSN: | 0021-9304 |
| Popis: | The morphology of progenitor endothelial cells on vascular graft surfaces is addressed in this report. Such cells were seen to attach to intima-expressed CD34 and Flk-1 antigen and showed positive 5-bromo-2-deoxyuridine (BrdU) uptake. We examined CD34 and Flk-1 antigen-expressing endothelial progenitor cells three-dimensionally using confocal laser scanning microscopy (CLSM). Under detailed CLSM observation, through an ameboid-form cell, these progenitor endothelial cells changed from a globular to a flattened form. We also investigated these morphological changes using scanning electron microscopy. From these results, progenitor endothelial cells were observed not only near the advancing edge of endothelium, but also around the developing intimal site. Their form also changed from globular to flattened as observed in the CLSM results. These morphological changes were seen more frequently near the advancing edge and around the developing intimal site. They attached directly to vascular prosthesis fibers and likewise covered the graft luminal surface. Progenitor endothelial cells in any form had a common surface structure. We conclude from our results that progenitor endothelial cells can attach to graft fibers directly without clotting and directly cover the graft luminal surfaces. |
| Databáze: | OpenAIRE |
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