Intraoperative collection of autologous platelet-rich plasma from the cardiopulmonary bypass circuit upon initiation of extracorporeal circulation
| Autor: | Yuji Kanaoka, Takeshi Honda, Yasuhiro Yunoki, Noriaki Kuwada, Taishi Tamura, Yoshiko Watanabe, Kazuo Tanemoto, Hiroshi Furukawa, Atsushi Tabuchi, Takahiko Yamasawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male medicine.medical_specialty Platelet Aggregation lcsh:Surgery law.invention lcsh:RD78.3-87.3 chemistry.chemical_compound Blood Transfusion Autologous Intraoperative Period law medicine Cardiopulmonary bypass Humans Platelet Cardiac Surgical Procedures Aged Cardiopulmonary Bypass biology business.industry Platelet Count Platelet-Rich Plasma Extracorporeal circulation lcsh:RD1-811 General Medicine Heparin Cardiac surgery Protamine Adenosine diphosphate surgical procedures operative chemistry Preoperative autologous platelet collection lcsh:Anesthesiology Cardiothoracic surgery Anesthesia biology.protein Surgery Platelet function Female Cardiology and Cardiovascular Medicine business medicine.drug Research Article |
| Zdroj: | Journal of Cardiothoracic Surgery Journal of Cardiothoracic Surgery, Vol 16, Iss 1, Pp 1-9 (2021) |
| ISSN: | 1749-8090 0000-2666 |
| Popis: | Objectives The aim of this study is to evaluate the possibility of the autologous platelet-rich plasma (PRP) collection from the cardiopulmonary bypass (CPB) circuit and to evaluate its effect on the aggregative function. Methods For seventy-two patients undergoing cardiac surgery with CPB, an autologous PRP was prepared using the Haemonetics Component Collection System® by drawing blood from the CPB circuit immediately after CPB was established. The blood samples were taken at three points for examination, A: beginning of surgery, B: immediately after heparin reversal with protamine following discontinuation of CPB, C: after the collected autologous PRP was returned to the patient. Platelet count and platelet aggregation ability were analyzed. Results The mean platelet count in autologous PRP was 5.5 (range: 3–14) units. Platelet count decreased by 115.0 (±27.3) × 1000/μl from A to B and increased by 27.3 ± 17.2 (× 1000/μl) from B to C. When platelet aggregation was measured by Adenosine Diphosphate (ADP) 3.0 μM, it decreased by 42.6% ± 12.1% from A to B and increased by 8.7% ± 7.4% from B to C. Conclusions Autologous PRP can be safely collected by drawing blood from the CPB circuit, platelet count and aggregation ability significantly decreased after CPB including autologous PRP collection. Some improvement was detected in the number of the platelets count and platelet aggregation ability by administrating an autologous PRP even if autologous PRP is collected from CPB circuit. Trial registration UMI-CTR, UMIN000023776. Registered 1 October 2016. |
| Databáze: | OpenAIRE |
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