Clinicopathologic significance of immunophenotypic profiles related to germinal center and activation B-cell differentiation in diffuse large B-cell lymphoma from Chinese patients
| Autor: | Xin lan Luo, Kin Chung Chris Lai, Li Li, Fen Zhang, Dong lan Luo, Dan Xie, Jie Xu, Hiu Ming Li, Fang Ping Xu, Heng guo Zhuang, Yan Hui Liu, Ming Hui Zhang |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male China Pathology medicine.medical_specialty Adolescent Population Biology Immunophenotyping Pathology and Forensic Medicine hemic and lymphatic diseases Biomarkers Tumor medicine Humans Child education Cyclin D3 B cell Aged Cell Proliferation Neoplasm Staging Retrospective Studies Aged 80 and over B-Lymphocytes education.field_of_study Tissue microarray Germinal center Middle Aged Germinal Center medicine.disease Lymphoma Survival Rate Cell Transformation Neoplastic medicine.anatomical_structure Tissue Array Analysis Child Preschool Female Lymph Nodes Lymphoma Large B-Cell Diffuse Diffuse large B-cell lymphoma |
| Zdroj: | Human Pathology. 39:875-884 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/j.humpath.2007.10.013 |
| Popis: | Diffuse large B-cell lymphoma (DLBCL) can be subdivided into prognostically significant groups with germinal center B-cell-like (GCB), activated B-cell-like (ABC), and type 3 groups. In this study, tissue microarray slides composed of 163 de novo DLBCLs from Chinese patients were immunostained for CD20, CD10, Bcl-6, MUM1, CD138, Bcl-2, Ki-67, cyclin D3, geminin, and P27(Kip1). One hundred forty-nine of 163 DLBCLs could then be classified into GCB group (pattern A), activated GCB group (pattern B) and activated non-GCB group (pattern C) according to the expression of CD10, Bcl-6, MUM1, and CD138. Of the 149 cases, 40 (26%) showed pattern A expression and were grouped as GCB group, lower than reported frequency of the studies involving mostly Western population. Compared with cases with pattern A, those with pattern B (activated GCB group) and C (activated non-GCB group) more often presented with more aggressive tumors and a shorter survival time. These results indicate that most of DLBCLs from Chinese patients can be classified into prognostically different groups based on the antigenic expression models using a panel of GCB- and ABC-associated markers. Polymerase chain reaction analysis of t(14;18) showed that 11 of 64 cases were t(14;18)-positive, and most (10 of 11) of it occurred in the group with pattern A. The translocation was significantly associated with expression of Bcl-2 protein. The group with pattern B demonstrated more frequent expression of Ki-67, cyclin D3, geminin, and showed higher proliferative activity than the group with pattern A. These findings suggest that high proliferative activity of tumors with pattern B may be associated with aggressive tumor behavior and poor clinical outcome in patients with DLBCL. |
| Databáze: | OpenAIRE |
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