SVEP1 as a Genetic Modifier of TEK-Related Primary Congenital Glaucoma
| Autor: | Nickie Stangel, Yongwook Dan, Conceição Egas, Terri L. Young, Kristina N. Whisenhunt, Jacob S Martin, Fatemeh Suri, Reza Maroofian, Samuel J Huang, Susana Carmona, Susan E. Quaggin, Tomokazu Souma, Emmanuelle Souzeau, Behzad Fallahi Motlagh, Jing Jin, Sarah M LaMartina, Nicole M Jody, Brendan M Lawson, Mehrnaz Narooie-Nejad, Heather D. Potter, Emily C. Higuchi, Owen M. Siggs, Jamie E Craig, Eduardo Silva, Vachiranee Limviphuvadh, Sebastian Maurer-Stroh, Xue Zhang, Elahe Elahi, Yasmin S. Bradfield, Maria José Simões, Evie Anagnos, Sean Martin, Stuart W. Tompson |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Pathology genetic structures Genotyping Techniques Glaucoma Penetrance Mice 0302 clinical medicine Gene Frequency Missense mutation Protein Isoforms Phosphorylation Exome sequencing Middle Aged Receptor TIE-2 Pedigree Schlemm's canal Child Preschool Female Haploinsufficiency medicine.medical_specialty Blotting Western Mutation Missense Biology 03 medical and health sciences Dysgenesis Exome Sequencing medicine Genetics Human Umbilical Vein Endothelial Cells Animals Humans Allele Allele frequency SVEP1 Intraocular Pressure Aged modifier Genes Modifier Hydrophthalmos Infant Newborn Infant medicine.disease 030104 developmental biology glaucoma HEK293 Cells TEK 030221 ophthalmology & optometry Cell Adhesion Molecules |
| Zdroj: | Investigative Ophthalmology & Visual Science |
| ISSN: | 1552-5783 0146-0404 |
| Popis: | Purpose Affecting children by age 3, primary congenital glaucoma (PCG) can cause debilitating vision loss by the developmental impairment of aqueous drainage resulting in high intraocular pressure (IOP), globe enlargement, and optic neuropathy. TEK haploinsufficiency accounts for 5% of PCG in diverse populations, with low penetrance explained by variable dysgenesis of Schlemm's canal (SC) in mice. We report eight families with TEK-related PCG, and provide evidence for SVEP1 as a disease modifier in family 8 with a higher penetrance and severity. Methods Exome sequencing identified coding/splice site variants with an allele frequency less than 0.0001 (gnomAD). TEK variant effects were assayed in construct-transfected HEK293 cells via detection of autophosphorylated (active) TEK protein. An enucleated eye from an affected member of family 8 was examined via histology. SVEP1 expression in developing outflow tissues was detected by immunofluorescent staining of 7-day mouse anterior segments. SVEP1 stimulation of TEK expression in human umbilical vascular endothelial cells (HUVECs) was measured by TaqMan quantitative PCR. Results Heterozygous TEK loss-of-function alleles were identified in eight PCG families, with parent-child disease transmission observed in two pedigrees. Family 8 exhibited greater disease penetrance and severity, histology revealed absence of SC in one eye, and SVEP1:p.R997C was identified in four of the five affected individuals. During SC development, SVEP1 is secreted by surrounding tissues. SVEP1:p.R997C abrogates stimulation of TEK expression by HUVECs. Conclusions We provide further evidence for PCG caused by TEK haploinsufficiency, affirm autosomal dominant inheritance in two pedigrees, and propose SVEP1 as a modifier of TEK expression during SC development, affecting disease penetrance and severity. |
| Databáze: | OpenAIRE |
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