Simultaneous Determination of 6 Antiretroviral Drugs in Human Hair Using an LC-ESI+-MS/MS Method: Application to Adherence Assessment
| Autor: | Shan Qiao, Zhiyong Shen, Liuxi Chu, Shuaifeng Liu, Haoran Yang, Huihua Deng, Xiaoming Li, Yuejiao Zhou, Wei Wang, Yan Wu, Quan Zhang, Jin Yang |
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| Rok vydání: | 2021 |
| Předmět: |
Efavirenz
Nevirapine HIV Infections Pharmacology Tandem mass spectrometry Article chemistry.chemical_compound Zidovudine Tandem Mass Spectrometry Humans Medicine Pharmacology (medical) Chromatography High Pressure Liquid business.industry Selected reaction monitoring Reproducibility of Results Lamivudine Lopinavir Pharmaceutical Preparations chemistry Ritonavir business Chromatography Liquid Hair medicine.drug |
| Zdroj: | Ther Drug Monit |
| ISSN: | 0163-4356 |
| DOI: | 10.1097/ftd.0000000000000878 |
| Popis: | BACKGROUND: The determination of antiretroviral drugs in hair is receiving considerable research interest to assess long-term adherence to antiretroviral therapy (ART). Currently in China, lamivudine, zidovudine, nevirapine, efavirenz, ritonavir, and lopinavir are combined as first- and second-line free therapy regimens and are recommended for people living with human immunodeficiency virus (HIV) (PLWH). Simultaneous determination of the six antiretroviral drugs in human hair is important for accurately and widely assessing long-term adherence in Chinese PLWH receiving different ART regimens. METHODS: Six drugs were extracted from 10-mg hair samples incubated in methanol for 16 h at 37 °C and then analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a mobile phase of 95% methanol, with an electrospray ionization (ESI) source in multiple reaction monitoring and positive mode. RESULTS: The LC-ESI(+)-MS/MS method exhibited a linear range (R(2) > 0.99) within 6–5000, 10–5000, 6–50000, 12–50000, 8–5000, and 8–12500 pg/mg for lamivudine, zidovudine, nevirapine, efavirenz, ritonavir, and lopinavir. For all six drugs, the limits of quantification ranged between 6 and 12 pg/mg. The intra-day and inter-day coefficients of variation were within 15%, and the recoveries ranged from 91.1% to 113.7%. Furthermore, the other validation parameters (i.e., selectivity, matrix effect, stability, and carryover) met the acceptance criteria stipulated by guidelines of the United States Food and Drug Administration and European Medicines Agency. Significant intergroup differences were observed between high- and low-adherence groups, with high inter-correlations in the hair content of the six drugs. CONCLUSIONS: The developed method demonstrated good reliability, to comprehensively and accurately assess adherence in PLWH receiving different ART regimens. |
| Databáze: | OpenAIRE |
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