The key differentially expressed genes and proteins related to immune response in the spleen of pufferfish (Takifugu obscurus) infected by Aeromonas hydrophila
| Autor: | Jianmin Ye, Xiufang Wei, Mingmei Ding, Meng Chen, QingJian Liang, An-Li Wang, Yanjian Yang, Shaoan Liao, Shengli Fu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine Proteome Down-Regulation Spleen Aquatic Science Microbiology Transcriptome Fish Diseases 03 medical and health sciences Immune system medicine Animals Environmental Chemistry KEGG Gene biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Aeromonas hydrophila Takifugu Up-Regulation 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries Signal transduction Gram-Negative Bacterial Infections |
| Zdroj: | Fish & Shellfish Immunology. 91:1-11 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2019.05.016 |
| Popis: | The immune mechanism elicited in pufferfish (Takifugu obscurus) against the invasion of Aeromonas hydrophila is still poorly understood. We examined the spleen of pufferfish at the transcriptome and proteome levels by using Illumina-seq and TMT coupled mass spectrometry after 12 h infection by A. hydrophila, respectively. A total of 2,339 genes (1,512 up-regulated and 827 down-regulated) and 537 (237 up-regulated and 300 down-regulated) proteins were identified. GO and KEGG analyses revealed that the responses to stimulus were the main biological processes, intestinal immune network for IgT production and calcium signaling pathway. Fourteen genes (8 up-regulated and 6 down-regulated) and proteins (5 up-regulated and 9 down-regulated) involved immune responses or signal transduction were validated by qRT-PCR and parallel reaction monitoring to confirm the reliability of the transcriptomic and proteomic analyses, respectively. Moreover, qRT-PCR and flow cytometry were used to detect dynamics of the genes in calcium signaling pathway and changes of concentration of cytoplasm Ca2+ in spleen cells within a 72 h challenge. This study provides the findings regarding immune response, especially intestinal immune network for IgT production pathway and calcium signaling pathway at the molecular, protein and cellular in pufferfish after infection by A. hydrophila. These results would provide a new insight and molecular targets into the response to pathogenic infection in pufferfish. |
| Databáze: | OpenAIRE |
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