Specific expression of secretogranin II in magnocellular vasopressin neurons of the rat supraoptic and paraventricular nucleus in response to osmotic stimulation
| Autor: | M. A. F. Sonnemans, C.H Dotman, F.W. van Leeuwen, C.W Ang, Reiner Fischer-Colbrie, Hans Winkler |
|---|---|
| Přispěvatelé: | Academic Medical Center |
| Rok vydání: | 1997 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Vasopressin Vasopressins Basal Ganglia Supraoptic nucleus Internal medicine Lactation Chromogranins medicine Animals Rats Wistar Molecular Biology Neurons Paraffin Embedding biology Chemistry General Neuroscience Neuropeptides Osmolar Concentration Proteins biology.organism_classification Axons Brattleboro rat Rats medicine.anatomical_structure Endocrinology nervous system Oxytocin Hypothalamus Protein Biosynthesis Magnocellular cell Female Neurology (clinical) Colchicine Supraoptic Nucleus Nucleus hormones hormone substitutes and hormone antagonists Paraventricular Hypothalamic Nucleus Developmental Biology medicine.drug |
| Zdroj: | Brain research, 765(1), 13-20. Elsevier |
| ISSN: | 0006-8993 |
| Popis: | As secretogranin II is considered to be a marker for the regulated secretory pathway, its distribution in the hypothalamo-neurohypophyseal system of salt-loaded Wistar rats was studied in detail by immunocytochemistry. Although after an osmotic challenge both vasopressin and oxytocin neurons are stimulated, secretogranin II was exclusively expressed in a subpopulation of vasopressinergic magnocellular neurons in the supraoptic and paraventricular nucleus of Wistar rats. Secretogranin II was only surely visualized after a combination of osmotic challenge and blockade of axonal transport by colchicine treatment. When these pre-treatments were not performed, only punctate fibers situated around the magnocellular neurons within the paraventricular and supraoptic nucleus were observed. Oxytocinergic magnocellular neurons never displayed any secretogranin II immunoreactivity, not even during lactation and after colchicine treatment. These findings suggest that secretogranin II is of functional importance during enhanced secretory activity within vasopressinergic neurons. |
| Databáze: | OpenAIRE |
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