The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning
| Autor: | Harald Saumweber, Man Mohan, Nina Heinl, Robert A. H. White, Rainer Renkawitz, Joerg Leers, Angela Philippen, Imke Bardenhagen, Martin Herold, Marek Bartkuhn, Renate Renkawitz-Pohl |
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| Rok vydání: | 2007 |
| Předmět: |
CCCTC-Binding Factor
Insulator (genetics) Article General Biochemistry Genetics and Molecular Biology Animals Drosophila Proteins Hox gene Enhancer Molecular Biology Body Patterning Homeodomain Proteins Genetics General Immunology and Microbiology biology General Neuroscience Gene Expression Regulation Developmental Nuclear Proteins biology.organism_classification DNA-Binding Proteins Repressor Proteins Drosophila melanogaster Enhancer Elements Genetic Phenotype CTCF Larva Bithorax complex Mutation Insulator Elements Homeotic gene Microtubule-Associated Proteins Drosophila Protein Protein Binding |
| Zdroj: | The EMBO Journal. 26:4203-4214 |
| ISSN: | 1460-2075 0261-4189 |
| DOI: | 10.1038/sj.emboj.7601851 |
| Popis: | Insulator sequences guide the function of distantly located enhancer elements to the appropriate target genes by blocking inappropriate interactions. In Drosophila, five different insulator binding proteins have been identified, Zw5, BEAF-32, GAGA factor, Su(Hw) and dCTCF. Only dCTCF has a known conserved counterpart in vertebrates. Here we find that the structurally related factors dCTCF and Su(Hw) have distinct binding targets. In contrast, the Su(Hw) interacting factor CP190 largely overlapped with dCTCF binding sites and interacts with dCTCF. Binding of dCTCF to targets requires CP190 in many cases, whereas others are independent of CP190. Analysis of the bithorax complex revealed that six of the borders between the parasegment specific regulatory domains are bound by dCTCF and by CP190 in vivo. dCTCF null mutations affect expression of Abdominal-B, cause pharate lethality and a homeotic phenotype. A short pulse of dCTCF expression during larval development rescues the dCTCF loss of function phenotype. Overall, we demonstrate the importance of dCTCF in fly development and in the regulation of abdominal segmentation. |
| Databáze: | OpenAIRE |
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