Real-time QCM-D monitoring of endothelial cells and macrophages adhering and spreading to SEMA4D/heparin surfaces
| Autor: | Wei Lai, Hongli Bai, Qiang Song, Junying Chen, Nan Huang, Jianying Tan, Zeng Zheng, Feng Zhou, Yuan Yuan Cui |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Time Factors Surface Properties Semaphorins 02 engineering and technology 03 medical and health sciences Colloid and Surface Chemistry Antigens CD Cell Adhesion medicine Humans Macrophage Particle Size Physical and Theoretical Chemistry Integrin binding Heparin Chemistry Macrophages Endothelial Cells Biomaterial Surfaces and Interfaces General Medicine Adhesion Quartz crystal microbalance 021001 nanoscience & nanotechnology In vitro Endothelial stem cell 030104 developmental biology Quartz Crystal Microbalance Techniques Biophysics 0210 nano-technology Biotechnology medicine.drug |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 171:522-529 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2018.07.062 |
| Popis: | In vitro evaluation techniques that address cellular interactions under dynamic circumstances are required for the development of advanced biomaterials. In this study, quartz crystal microbalance and dissipation (QCM-D) provided a surface sensitive technique that enabled real-time monitoring of the adhesion and spreading response of endothelial cells (ECs) and macrophages (MAs) to soluble-semaphoring 4D (SEMA4D)/heparin modified substratum. The high heparin and low SEMA4D of substratum promoted ECs adhering and spreading. However, surfaces with high SEMA4D and low heparin enhanced MA adhering and spreading. Furthermore, DF plots of QCM-D indicated that the adhering and spreading of MAs were mainly mediated via the ligand-receptor interaction of SEMA4D-CD72, while both adhering and spreading of ECs were mainly mediated via heparin-induced integrin binding. This study suggests that QCM-D combined with other appropriate methods can be utilized to explore the mechanisms for cellular interaction with biomaterial surfaces. |
| Databáze: | OpenAIRE |
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