Taking Two-Photon Excitation to Exceptional Path-Lengths in Photonic Crystal Fiber
| Autor: | Anita C. Jones, Tijmen G. Euser, Gareth O. S. Williams, Jochen Arlt, Philip St. J. Russell |
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| Přispěvatelé: | Euser, Tijmen [0000-0002-8305-9598], Apollo - University of Cambridge Repository |
| Rok vydání: | 2019 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Materials science photochemistry business.industry optofluidics nonlinear optics Nonlinear optics Physics::Optics two-photon cross-section Two-photon absorption Atomic and Molecular Physics and Optics Optofluidics Electronic Optical and Magnetic Materials Optics Nanolithography Two-photon excitation microscopy Optoelectronics two-photon absorption fluorescence Electrical and Electronic Engineering business Excitation Biotechnology Photonic-crystal fiber |
| Zdroj: | Williams, G O S, Euser, T G, Arlt, J, Russell, P S J & Jones, A C 2014, ' Taking Two-Photon Excitation to Exceptional Path-Lengths in Photonic Crystal Fiber ', ACS Photonics, vol. 1, no. 9, pp. 790-793 . https://doi.org/10.1021/ph5002236 |
| DOI: | 10.17863/cam.40551 |
| Popis: | The well-known, defining feature of two-photon excitation (TPE) is the tight, three-dimensional confinement of excitation at the intense focus of a laser beam. The extremely small excitation volume, on the order of 1 μm3 (1 femtoliter), is the basis of far-reaching applications of TPE in fluorescence imaging, photodynamic therapy, nanofabrication, and three-dimensional optical memory. Paradoxically, the difficulty of detecting photochemical events in such a small volume is a barrier to the development of the two-photon-activated molecular systems that are essential to the realization of such applications. We show, using two-photon-excited fluorescence to directly visualize the excitation path, that confinement of both laser beam and sample solution within the 20 μm hollow core of a photonic crystal fiber permits TPE to be sustained over an extraordinary path-length of more than 10 cm, presenting a new experimental paradigm for ultrasensitive studies of two-photon-induced processes in solution. (Figure Presented). We are grateful to the Koerber Foundation (Germany) and the EPSRC (UK) for financial support. G.O.S.W. is a recipient of an EPSRC Prize Postdoctoral Fellowship |
| Databáze: | OpenAIRE |
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