Drosophila mojoless, a Retroposed GSK-3, Has Functionally Diverged to Acquire an Essential Role in Male Fertility
| Autor: | Esther Siegfried, Brian Oliver, Rasika Kalamegham, David Sturgill |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Male
Retroelements Somatic cell Immunoblotting Molecular Sequence Data Germline Article Glycogen Synthase Kinase 3 Gene duplication Testis Genetics medicine Animals Cluster Analysis Drosophila Proteins Amino Acid Sequence Selection Genetic Molecular Biology Gene Ecology Evolution Behavior and Systematics DNA Primers Likelihood Functions Microscopy Confocal biology Base Sequence Models Genetic Sequence Analysis DNA biology.organism_classification medicine.anatomical_structure Fertility Germ Cells Drosophila RNA Interference Drosophila melanogaster Sequence Alignment Germ cell Functional divergence Drosophila Protein |
| Popis: | Retroposition is increasingly recognized as an important mechanism for the acquisition of new genes. We show that a glycogen synthase kinase-3 gene, shaggy (sgg), retroposed at least 50 MYA in the Drosophila genus to generate a new gene, mojoless (mjl). We have extensively analyzed the function of mjl and examined its functional divergence from the parental gene sgg in Drosophila melanogaster. Unlike Sgg, which is expressed in many tissues of both sexes, Mjl is expressed specifically in the male germ line, where it is required for male germ line survival. Our analysis indicates that mjl has acquired a specific function in the maintenance of male germ line viability. However, it has not completely lost its ancestral biochemical function and can partially compensate for loss of the parental gene sgg when ectopically expressed in somatic cells. We postulate that mjl has undergone functional diversification and is now under stabilizing selection in the Drosophila genus. |
| Databáze: | OpenAIRE |
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