Granulocyte differentiation inducer, hexapeptide HLDF-6, decreases cytotoxic effect of tumor necrosis factor on HL-60 cell line
| Autor: | Tatiana V. Rakitina, Valery M. Lipkin, Irina A. Kostanyan, N. V. Gibanova |
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| Rok vydání: | 2007 |
| Předmět: |
Ceramide
Cellular differentiation Biophysics Apoptosis HL-60 Cells Biology Ceramides Biochemistry Biochemistry Genetics and Molecular Biology (miscellaneous) Monocytes chemistry.chemical_compound Serum response factor Humans Cytotoxic T cell Drug Interactions Caspase 8 Caspase 3 Tumor Necrosis Factor-alpha NF-kappa B Cell Differentiation General Medicine Molecular biology chemistry Monocyte differentiation Tumor necrosis factor alpha Geriatrics and Gerontology Oligopeptides Granulocytes Signal Transduction Differentiation Inducer |
| Zdroj: | Biochemistry (Moscow). 72:49-60 |
| ISSN: | 1608-3040 0006-2979 |
| Popis: | The effect of hexapeptide HLDF-6, the granulocytic differentiation inducer, on the tumor necrosis factor alpha (TNF-alpha)-induced differentiation and apoptosis of human promyelocytic leukemia HL-60 cells has been investigated. Costimulation of HL-60 cells with HLDF-6 and TNF-alpha enhanced granulocyte differentiation, whereas the level of monocyte differentiation remained unchanged; however, the cytotoxic action of TNF-alpha on these cells decreased. The protective effect of HLDF-6 peptide did not depend on activation of NF-kappaB (nuclear transcription factor). Since HLDF-6 peptide decreases the number of cells entering apoptosis caused by C(2)-ceramide, a mediator of TNF-induced apoptosis, and also reduces TNF-alpha-mediated activation of caspase-3, we have proposed the hypothesis that HLDF-6 increases resistance of HL-60 cells to the TNF-alpha cytotoxic effect due to inhibition of some stages of mitochondria-dependent apoptotic signaling. |
| Databáze: | OpenAIRE |
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