Analysis of the circadian clock gene period in the sheep blow fly Lucilia cuprina
| Autor: | Guy R. Warman, Richard D. Newcomb, R. D. Lewis, C. W. Evans |
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| Rok vydání: | 2000 |
| Předmět: |
Repetitive Sequences
Amino Acid Threonine DNA Complementary Time Factors Sequence analysis Molecular Sequence Data Glycine Genes Insect Biological Clocks Complementary DNA Genetics Animals Drosophila Proteins Amino Acid Sequence RNA Messenger Circadian rhythm Amino Acids Oscillating gene Gene Phylogeny Chronobiology Sheep Base Sequence biology Diptera fungi Nuclear Proteins Period Circadian Proteins General Medicine biology.organism_classification Molecular biology Bacterial circadian rhythms Circadian Rhythm Lucilia cuprina Insect Proteins |
| Zdroj: | Genetical Research. 75:257-267 |
| ISSN: | 1469-5073 0016-6723 |
| DOI: | 10.1017/s0016672399004425 |
| Popis: | We have isolated a homologue of the period (per) gene from the Australian sheep blow fly, Lucilia cuprina, as part of a comparative approach to the analysis of dipteran circadian systems. Sequence analysis of the 4 kb per cDNA revealed the conservation of three functional domains, namely the PAS dimerization motif, and the nuclear and cytoplasmic localization domains. A fourth domain, the threonine–glycine (TG) repeat region, is also conserved in L. cuprina per but has been severely truncated. No length variation was found in the TG repeat of L. cuprina or L. sericata collected from several different latitudinal zones. Expression analysis indicated a diel oscillation in per mRNA in LD 12[ratio ]12 with a period of 24 h and a peak at Zt 12. PER-immunoreactive protein oscillations were also demonstrated, with peak immunoreactivity lagging approximately 3 h behind peak mRNA levels. These results show the existence of a Drosophila-like circadian system in a calliphorid fly. They also provide evidence for the conservation of per function across the Diptera, and confirm the relevance of the Drosophila system as a model for fly circadian rhythms. |
| Databáze: | OpenAIRE |
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