USP1 (ubiquitin specific peptidase 1) targets ULK1 and regulates its cellular compartmentalization and autophagy

Autor: Francesco La Marra, Francesca Demarchi, Marzia Raimondi, Claudio Schneider, Daniela Cesselli, Carla Di Loreto
Rok vydání: 2018
Předmět:
0301 basic medicine
Cell Survival
Research Paper - Basic Science
Breast Neoplasms
macromolecular substances
Deubiquitinating enzyme
03 medical and health sciences
Mice
breast cancer
Ubiquitin
Sequestosome-1 Protein
Autophagy
Animals
Autophagy-Related Protein-1 Homolog
Humans
Phosphorylation
Molecular Biology
MAP1LC3B
Aggresomes
noncanonical autophagy
030102 biochemistry & molecular biology
biology
deubiquitinating enzymes
Kinase
Intracellular Signaling Peptides and Proteins
Ubiquitination
Cell Biology
Compartmentalization (psychology)
ULK1
Fibroblasts
pimozide
Cell biology
Cell Compartmentation
030104 developmental biology
Aggresome
HEK293 Cells
nervous system
biology.protein
Beclin-1
Female
Ubiquitin-Specific Proteases
Apoptosis Regulatory Proteins
Microtubule-Associated Proteins
Protein Processing
Post-Translational
Signal Transduction
Zdroj: Autophagy. 15(4)
ISSN: 1554-8635
Popis: ULK1 (unc-51 like autophagy activating kinase 1) is a core component at multiple steps of canonical macroautophagy/autophagy. The activity of ULK1 is tightly regulated by several post-translational modifications, including ubiquitination, yet the deubiquitinase (DUB) responsible for its reversible deubiquitination has not been described. Here, we identified USP1 (ubiquitin specific peptidase 1) as a key player in the modulation of ULK1 K63-linked deubiquitination. Moreover, both USP1 depletion and its chemical inhibition by pimozide are coupled to a reduction of ULK1 in Triton X-100 soluble cellular lysates, and its compartmentalization to a fraction that can be solubilized in 5 M urea. In USP1-depleted cells this fraction is also enriched in SQSTM1 (sequestosome 1), the aggresome marker HDAC6 (histone deacetylase 6), and the prototype of USP1 targets FANCD2 (FA complementation group D2). Consistently, in USP1-depleted and pimozide-treated cells, ULK1 forms protein aggregates enriched in SQSTM1, as detected by both immummunofluorescence and co-immunoprecipitation studies. Notably, depletion of USP1 inhibits canonical autophagic flux and promotes an alternative route leading to lysosomal-mediated degradation of SQSTM1. Our findings reveal a novel function of the USP1-ULK1 axis as a modulator of the switch between canonical and unconventional autophagy. Further, we provide the first evidence supporting the existence of a subset of breast tumors co-expressing ULK1 and MAP1LC3B (microtubule associated protein 1 light chain 3 beta) proteins. Because the USP1 inhibitor pimozide affects breast cancer cell growth, targeting USP1 in those tumors relying on autophagy for growth might prove to be a convenient therapeutic strategy. Abbreviations: ATG13: autophagy related 13; BECN1: beclin 1; BZ: bortezomib; CAPN1: calpain 1; DUB: deubiquitinase; FANCI: FA complementation group I; FANCD2: FA complementation group D2; FZR1: fizzy and cell division cycle 20 related 1; HDAC6: histone deacetylase 6; MAP1LC3B: microtubule associated protein 1 light chain 3 beta; PMZ: pimozide; SH3GLB1: SH3 domain containing GRB2 like, endophilin B1; SQSTM1: sequestosome 1; TRAF6: TNF receptor associated factor 6; ULK1: unc-51 like autophagy activating kinase 1; USP1: ubiquitin specific peptidase 1; WDR48: WD repeat domain 48.
Databáze: OpenAIRE
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