Up-regulation of microtubule-associated protein 2 accompanying the filial imprinting of domestic chicks (Gallus gallus domesticus)
| Autor: | Koichi J. Homma, Tatsuya Takano, Shinji Yamaguchi, Ikuko Fujii-Taira, Akio Murakami, Naoki Hirose, Yasuyuki Fujimoto, Toshiya Matsushima, Ei Ichi Izawa, Naoya Aoki |
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| Rok vydání: | 2007 |
| Předmět: |
Neurons
Messenger RNA animal structures General Neuroscience Hippocampus Brain In situ hybridization Imprinting Psychological Biology Molecular biology Cell biology Up-Regulation Cytosol Microtubule-associated protein 2 Gene Expression Regulation Microtubule Gene expression Animals RNA Messenger Gene Chickens Microtubule-Associated Proteins In Situ Hybridization Oligonucleotide Array Sequence Analysis |
| Zdroj: | Brain research bulletin. 76(3) |
| ISSN: | 1873-2747 |
| Popis: | Using cDNA microarrays, we have identified elsewhere the genes of microtubule-associated proteins as a group up-regulated in newly hatched chick brains after filial imprinting training. Here we show by in situ hybridization that the mRNA for the microtubule-associated protein 2 (MAP2) gene was enriched in the mesopallium and the hippocampus in the trained chick brain. The regionally specific enrichments of MAP2 mRNA were not observed in the brain of dark-reared or light-exposed chick as controls, implying an association between the degree of expression and the strength of the learned preference. In agreement with the gene expression, MAP2 protein was accumulated in the mesopallium of the trained chick brain, but not in the brains of the controls. The accumulation of MAP2 was found in the cytosol of neurons and co-localized with beta-tubulin, suggesting a change in microtubule assembly. Our results suggest a postnatal reorganization of cytoskeleton following filial imprinting. |
| Databáze: | OpenAIRE |
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