Identification of forensically important flesh flies based on a shorter fragment of the cytochrome oxidase subunit I gene in China
| Autor: | Jifang Wen, Yadong Guo, Yunfeng Chang, L. Liang, F. M. Meng, Lingmei Lan, Jifeng Cai, Yan Gu |
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| Rok vydání: | 2011 |
| Předmět: |
China
Mitochondrial DNA Protein subunit Molecular Sequence Data Sarcophagidae Biology DNA Mitochondrial Polymerase Chain Reaction Electron Transport Complex IV Species Specificity Animals DNA Barcoding Taxonomic Cytochrome c oxidase Forensic entomology Gene Phylogeny Ecology Evolution Behavior and Systematics Genetics Oxidase test General Veterinary Phylogenetic tree Forensic Sciences Sequence Analysis DNA Insect Science biology.protein Insect Proteins Parasitology Identification (biology) Entomology Sequence Alignment |
| Zdroj: | Medical and Veterinary Entomology. 26:307-313 |
| ISSN: | 0269-283X |
| DOI: | 10.1111/j.1365-2915.2011.01003.x |
| Popis: | With the development of molecular identification, there has been a great deal of discussion about the feature of the mitochondrial DNA (mtDNA) fragments. Although longer fragments may minimize stochastic variation across taxa and be more likely to reflect broader patterns of nucleotide divergence, shorter fragments have many advantages, such as quick, easy and economical. Extensive application of long mtDNA segments for species identification cannot always be achieved as a result of constraints in time and money. In the present study, a molecular identification method involving the sequencing of a 272-bp 'barcode' fragment of the mitochondrial cytochrome oxidase subunit I (COI) gene from 55 specimens, representing 7 Chinese sarcophagid species from varying populations, was evaluated. Phylogenetic analysis of the sequenced segments showed that all sarcophagid specimens were properly assigned into seven species, which indicated the possibility of separation congeneric species with the short fragments. The results of this research will be instrumental for the implementation of the Chinese Sarcophagidae database. |
| Databáze: | OpenAIRE |
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