Human astrovirus isolation and propagation in multiple cell lines
| Autor: | Neil R. Blacklow, John E. Herrmann, James P. Brinker |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Serotype
Time Factors viruses Virus Astrovirus Cell Line HT29 Cells Feces fluids and secretions Virology Chlorocebus aethiops Animals Humans Fluorescent Antibody Technique Indirect Vero Cells biology virus diseases General Medicine Haplorhini biology.organism_classification Staining Cell culture Vero cell African Green Monkey Caco-2 Cells Mamastrovirus |
| Zdroj: | Archives of virology. 145(9) |
| ISSN: | 0304-8608 |
| Popis: | Laboratory adapted human astrovirus serotypes 1 through 7 were tested for growth in 15 human, 7 simian, and 10 other non-primate mammalian cell lines. Propagation of all seven serotypes was successful in the human cell lines Caco-2, T84, HT-29, and in the African green monkey kidney cell line MA-104. Both primary and secondary African green monkey kidney cells were more effective than Rhesus monkey kidney cells for cultivation of astrovirus. Except for human foreskin cells, all of the other human and simian cell lines supported growth of at least one astrovirus serotype. The only non-primate cell line that permitted sustained passage of astroviruses was the BHK-21 (C13) cell line for astrovirus serotype 2. Seventeen human stool specimens that had previously been shown to be astrovirus positive by ELISA were cultured in Caco-2, T84, HT-29, SK-CO-1, PLC/PRF/5, MA-104, and VERO cells. Caco-2 cells (13 isolates), T84 cells (12 isolates) and PLC/PRF/5 cells (12 isolates) were the cell lines most effective for isolation of human astroviruses from clinical stool specimens. By immunofluorescent staining of infected cells, culturing of the same 17 specimens in shell vials for 18 h was positive for astroviruses in all 17 specimens in Caco-2 cells, 12 in T84 cells, and 7 in PLC/PRF/5 cells. Shell vial assay is suitable as a rapid and sensitive culture technique for detection of astroviruses in clinical specimens. |
| Databáze: | OpenAIRE |
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