UVC irradiation suppresses platelet-derived growth factor-BB-induced migration in human pancreatic cancer cells
| Autor: | Seiji Adachi, Hisataka Moriwaki, Masahiko Itani, Takahiro Yamauchi, Junji Kawaguchi, Ichiro Yasuda, Osamu Kozawa, Takashi Yoshioka |
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| Rok vydání: | 2011 |
| Předmět: |
MAPK/ERK pathway
Cancer Research Time Factors cell migration Ultraviolet Rays p38 mitogen-activated protein kinases pancreatic cancer Becaplermin Biology p38 Mitogen-Activated Protein Kinases Glycogen Synthase Kinase 3 Cell Movement GSK-3 platelet-derived growth factor-BB Cell Line Tumor Humans Neoplasm Invasiveness Phosphorylation Protein kinase A Protein kinase B Mitogen-Activated Protein Kinase 1 Glycogen Synthase Kinase 3 beta Mitogen-Activated Protein Kinase 3 Kinase Cell growth Akt JNK Mitogen-Activated Protein Kinases Articles Proto-Oncogene Proteins c-sis General Medicine Molecular biology Recombinant Proteins Pancreatic Neoplasms Oncology Cancer research biology.protein UVC Proto-Oncogene Proteins c-akt Platelet-derived growth factor receptor Signal Transduction |
| Zdroj: | Oncology Reports |
| ISSN: | 1791-2431 1021-335X |
| DOI: | 10.3892/or.2011.1612 |
| Popis: | We have recently reported that short wavelength ultraviolet-C (UVC) irradiation inhibits cell growth and induces apoptosis in human pancreatic cancer cells. In this study, we investigated the effect of UVC on platelet-derived growth factor (PDGF)-BB-induced migration in pancreatic cancer cells, AsPC1 and BxPC3. In cell migration assays using a Boyden chamber Transwell, PDGF-BB exerted a maximum effect on migration of these cells at a dose of 70 ng/ml after 36 h of treatment. PDGF-BB also caused phosphorylation of p44/p42 mitogen-activated protein kinase (MAPK), stress-activated protein kinase/c-Jun-N-terminal kinase (SAPK/JNK) and Akt, but not of p38 MAPK in these cells. Pretreatment of these cells with UVC at a dose over 10 J markedly suppressed PDGF-BB-induced migration. Since UVC significantly inhibited PDGF-BB-induced phosphorylation of Akt, and subsequent glycogen synthase kinase (GSK) 3β, but not p44/p42 MAPK and SAPK/JNK, it is likely that UVC inhibits PDGF-BB-induced migration by suppressing the Akt-GSK3β pathway in pancreatic cancer cells. Taken together with our previous findings, UVC could be a useful tool for the treatment of patients with pancreatic cancer. |
| Databáze: | OpenAIRE |
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