Intrafollicular Concentrations of the Oocyte-secreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries
| Autor: | Stine Gry Kristensen, Ajay Kumar, Linn Salto Mamsen, Bhanu Kalra, Susanne Elisabeth Pors, Jane Alrø Bøtkjær, Kirsten Tryde Macklon, Jens Fedder, Erik Ernst, Kate Hardy, Stephen Franks, Claus Yding Andersen |
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| Přispěvatelé: | Genesis Research Trust |
| Rok vydání: | 2022 |
| Předmět: |
EXPRESSION
Anti-Mullerian Hormone BETA SUPERFAMILY Endocrinology Diabetes and Metabolism Inhibins/metabolism Clinical Biochemistry MUTATION ANALYSIS Growth Differentiation Factor 9 follicle fluid Biomarkers/analysis Biochemistry Endocrinology & Metabolism Endocrinology BMP15 Transforming Growth Factor beta PCOS Humans Inhibins Science & Technology Anti-Mullerian Hormone/analysis Polycystic Ovary Syndrome/diagnosis INHIBIN-B GROWTH-DIFFERENTIATION FACTOR-9 Oocytes/metabolism Biochemistry (medical) MORPHOGENETIC PROTEIN 15 Follicular Fluid/chemistry WOMEN 1103 Clinical Sciences GDF9 GRANULOSA-CELLS CUMULUS CELLS Follicular Fluid cumulin Oocytes 1114 Paediatrics and Reproductive Medicine Female Bone Morphogenetic Protein 15/analysis antral follicle MESSENGER-RNA Bone Morphogenetic Protein 15 Life Sciences & Biomedicine Transforming Growth Factor beta/metabolism Biomarkers Growth Differentiation Factor 9/analysis Polycystic Ovary Syndrome |
| Zdroj: | E3383 E3374 Kristensen, S G, Kumar, A, Mamsen, L S, Kalra, B, Pors, S E, Bøtkjær, J A, Macklon, K T, Fedder, J, Ernst, E, Hardy, K, Franks, S & Andersen, C Y 2022, ' Intrafollicular Concentrations of the Oocyte-secreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries ', The Journal of Clinical Endocrinology & Metabolism, vol. 107, no. 8, pp. e3374-e3383 . https://doi.org/10.1210/clinem/dgac272 Kristensen, S G, Kumar, A, Mamsen, L S, Kalra, B, Pors, S E, Bøtkjær, J A, Macklon, K T, Fedder, J, Ernst, E, Hardy, K, Franks, S & Andersen, C Y 2022, ' Intrafollicular Concentrations of the Oocytesecreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries ', Journal of Clinical Endocrinology and Metabolism, vol. 107, no. 8, pp. E3374-E3383 . https://doi.org/10.1210/clinem/dgac272 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/clinem/dgac272 |
| Popis: | Context The oocyte-secreted factors growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) play essential roles in follicle development and oocyte maturation, and aberrant regulation might contribute to the pathogenesis of polycystic ovary syndrome. Objective Are there measurable differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in small antral follicle fluids from women with and without polycystic ovaries (PCO)? Design and Setting Follicle fluids (n = 356) were collected from 4- to 11-mm follicles in unstimulated ovaries of 87 women undergoing ovarian tissue cryopreservation for fertility preservation. Patients Twenty-seven women with PCO were identified and 60 women without PCO-like characteristics (non-PCO women) were matched according to age and follicle size. Main outcome measures Intrafollicular concentrations of GDF9, BMP15, GDF9/BMP15 heterodimer, anti-Mullerian hormone (AMH), inhibin-A and -B, total inhibin, activin-B and -AB, and follistatin were measured using enzyme-linked immunosorbent assays. Results The detectability of GDF9, BMP15, and the GDF9/BMP15 heterodimer were 100%, 94.4%, and 91.5%, respectively, and concentrations were significantly negatively correlated with increasing follicle size (P Conclusions Intrafollicular concentrations of GDF9 and BMP15 varied inversely in women with PCO reflecting an aberrant endocrine environment. An increased GDF9:BMP15 ratio may be a new biomarker for PCO. |
| Databáze: | OpenAIRE |
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