Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing
| Autor: | Lei Zhao, Vojtech Adam, Tarja K. Nevanen, Emily P. Nguyen, Arben Merkoçi, Ondrej Zitka, Jiri Kudr, Henri O. Arola |
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| Rok vydání: | 2019 |
| Předmět: |
Biomedical Engineering
Biophysics Oxide 2D material 02 engineering and technology Biosensing Techniques 01 natural sciences law.invention Immunoenzyme Techniques chemistry.chemical_compound law Electrochemistry Antibody Graphene oxide Reagent Strips Detection limit Chemistry Graphene 010401 analytical chemistry Substrate (chemistry) General Medicine Electrochemical Techniques Equipment Design 021001 nanoscience & nanotechnology 0104 chemical sciences Microelectrode T-2 Toxin Electrode Graphite Differential pulse voltammetry 0210 nano-technology Biosensor Antibodies Immobilized Microelectrodes Oxidation-Reduction Biotechnology Nuclear chemistry |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Kudr, J, Zhao, L, Nguyen, E P, Arola, H, Nevanen, T K, Adam, V, Zitka, O & Merkoçi, A 2020, ' Inkjet-printed electrochemically reduced graphene oxide microelectrode as a platform for HT-2 mycotoxin immunoenzymatic biosensing ', Biosensors & Bioelectronics, vol. 156, 112109 . https://doi.org/10.1016/j.bios.2020.112109 Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) Recercat. Dipósit de la Recerca de Catalunya instname |
| ISSN: | 1873-4235 |
| DOI: | 10.1016/j.bios.2020.112109 |
| Popis: | The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 μm in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectrodes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectrode surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electroactive reporter – 1-N. The biosensor showed detection limit of 1.6 ng ∙ mL−1 and a linear dynamic range of 6.3 – 100.0 ng ∙ mL−1 within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate. |
| Databáze: | OpenAIRE |
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